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JAC Advance Access originally published online on January 13, 2005
Journal of Antimicrobial Chemotherapy 2005 55(2):260-264; doi:10.1093/jac/dkh541
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JAC vol.55 no.2 © The British Society for Antimicrobial Chemotherapy 2005; all rights reserved

Enhanced resistance to erythromycin is conferred by the enterococcal msrC determinant in Staphylococcus aureus

Elinor Reynolds and Jonathan H. Cove*

School of Biochemistry and Microbiology, University of Leeds, Leeds LS2 9JT, UK

* Corresponding author. Tel: +44-113-343-5630; Fax: +44-113-343-5638; Email: j.h.cove{at}leeds.ac.uk

Objectives:

The msrC gene, found on the chromosome of Enterococcus faecium, shares a high degree of similarity with the staphylococcal erythromycin resistance determinant msr(A). The enterococcal determinant was cloned into Staphylococcus aureus to determine whether msrC could confer antibiotic resistance in staphylococci.

Methods:

A shuttle vector comprising pBluescript and pSK265 was used to introduce multiple copies of msrC into S. aureus RN4220. The integration vector pCL84 was employed to insert a single copy of msrC into the S. aureus chromosome. MICs were determined by the broth microdilution method.

Results:

Expression of msrC from both chromosomal and plasmid loci in erythromycin-susceptible S. aureus RN4220 (MIC 0.25 mg/L) gave rise to enhanced protection against erythromycin, with an MIC of 32–64 mg/L for S. aureus RN4220 containing msrC in multiple copies and an MIC of 16–64 mg/L with msrC inserted as a single copy in the S. aureus chromosome.

Conclusions:

MsrC mediates high-level resistance to erythromycin in S. aureus.

Keywords: macrolide resistance , ABC transporter , molecular cloning , staphylococci , enterococci


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