JAC Advance Access originally published online on December 1, 2004
Journal of Antimicrobial Chemotherapy 2005 55(1):61-70; doi:10.1093/jac/dkh512
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
JAC vol.55 no.1 © The British Society for Antimicrobial Chemotherapy 2004; all rights reserved
Italian metallo-ß-lactamases: a national problem? Report from the SENTRY Antimicrobial Surveillance Programme
1 Department of Pathology & Microbiology, University of Bristol, Bristol, UK; 2 The JONES Group/JMI Laboratories, North Liberty, IA, USA
* Corresponding author. Tel: +44-117-9287522; Fax: +44-117-9287896; Email: mark.toleman{at}bristol.ac.uk
Objectives: As part of the SENTRY Antimicrobial Surveillance Programme, 383 non-replicative randomly collected Pseudomonas aeruginosa isolates were collected during 1999–2002. These strains originated from three geographically distinct hospitals within Italy: Genoa (Northern Italy); Rome and Catania (Sicily), and were further studied to identify the prevalence of metallo-ß-lactamase (MßL) alleles across Italy and to determine their genetic details.
Methods: Multidrug-resistant (MDR) strains were identified by MIC analysis followed by genotyping and PCR-based strategies.
Results: Initial MIC analysis identified 31 MDR isolates that displayed an Etest MßL-positive phenotype. Of these, 25 produced either the MßL VIM-1 or IMP-13 as detected by PCR and sequencing. VIM-1-producing isolates were found at all sites, whereas IMP-13-producing isolates were only found in Rome. MßL-producing isolates were found at all Italian SENTRY sites and together amounted to 6.5% of all P. aeruginosa isolates. Genetic analysis indicated that many strains contained multiple integrons and identified two novel MßL integrons, one from the site in Genoa and one from Sicily. Integrons identical in structure and sequence to In70, the first identified and characterized blaVIM-containing integron from Verona, were found in isolates with distinct ribotypes at the Roman and Sicilian sites indicating that this integron has recently disseminated across Italy. All 25 MßL-producing isolates were genetically linked in that all isolates contained Tn5051 sequences and all harboured the insertion sequence IsPa7 which may be involved in the mobilization of these resistance alleles.
Conclusions: Taken together, these results indicate that Italy has a nationwide problem of MDR P. aeruginosa produced by mobile MßL genes.
Keywords: Pseudomonas aeruginosa , IsPa7 , Tn5051 , integrons , Italy
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  R. C. Picao, S. S. Andrade, A. G. Nicoletti, E. H. Campana, G. C. Moraes, R. E. Mendes, and A. C. Gales Metallo-{beta}-Lactamase Detection: Comparative Evaluation of Double-Disk Synergy versus Combined Disk Tests for IMP-, GIM-, SIM-, SPM-, or VIM-Producing Isolates J. Clin. Microbiol., June 1, 2008; 46(6): 2028 - 2037. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Mazzariol, M. Aldegheri, M. Ligozzi, G. Lo Cascio, R. Koncan, and R. Fontana Performance of Vitek 2 in Antimicrobial Susceptibility Testing of Pseudomonas aeruginosa Isolates with Different Mechanisms of {beta}-Lactam Resistance J. Clin. Microbiol., June 1, 2008; 46(6): 2095 - 2098. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Aschbacher, M. Doumith, D. M. Livermore, C. Larcher, and N. Woodford Linkage of acquired quinolone resistance (qnrS1) and metallo-{beta}-lactamase (blaVIM-1) genes in multiple species of Enterobacteriaceae from Bolzano, Italy J. Antimicrob. Chemother., March 1, 2008; 61(3): 515 - 523. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Neuwirth, E. Siebor, F. Robin, and R. Bonnet First Occurrence of an IMP Metallo-{beta}-Lactamase in Aeromonas caviae: IMP-19 in an Isolate from France Antimicrob. Agents Chemother., December 1, 2007; 51(12): 4486 - 4488. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. A. Toleman, H. Vinodh, U. Sekar, V. Kamat, and T. R. Walsh blaVIM-2-Harboring Integrons Isolated in India, Russia, and the United States Arise from an Ancestral Class 1 Integron Predating the Formation of the 3' Conserved Sequence Antimicrob. Agents Chemother., July 1, 2007; 51(7): 2636 - 2638. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. E. Mendes, K. A. Kiyota, J. Monteiro, M. Castanheira, S. S. Andrade, A. C. Gales, A. C. C. Pignatari, and S. Tufik Rapid Detection and Identification of Metallo-{beta}-Lactamase-Encoding Genes by Multiplex Real-Time PCR Assay and Melt Curve Analysis J. Clin. Microbiol., February 1, 2007; 45(2): 544 - 547. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. D. D. Pitout, B. L. Chow, D. B. Gregson, K. B. Laupland, S. Elsayed, and D. L. Church Molecular Epidemiology of Metallo-{beta}-Lactamase-Producing Pseudomonas aeruginosa in the Calgary Health Region: Emergence of VIM-2-Producing Isolates J. Clin. Microbiol., February 1, 2007; 45(2): 294 - 298. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Ktari, G. Arlet, B. Mnif, V. Gautier, F. Mahjoubi, M. Ben Jmeaa, M. Bouaziz, and A. Hammami Emergence of Multidrug-Resistant Klebsiella pneumoniae Isolates Producing VIM-4 Metallo-{beta}-Lactamase, CTX-M-15 Extended-Spectrum {beta}-Lactamase, and CMY-4 AmpC {beta}-Lactamase in a Tunisian University Hospital Antimicrob. Agents Chemother., December 1, 2006; 50(12): 4198 - 4201. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Fiett, A. Baraniak, A. Mrowka, M. Fleischer, Z. Drulis-Kawa, L. Naumiuk, A. Samet, W. Hryniewicz, and M. Gniadkowski Molecular Epidemiology of Acquired-Metallo-{beta}-Lactamase-Producing Bacteria in Poland Antimicrob. Agents Chemother., March 1, 2006; 50(3): 880 - 886. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. G. Gacar, K. Midilli, F. Kolayli, K. Ergen, S. Gundes, S. Hosoglu, A. Karadenizli, and H. Vahaboglu Genetic and Enzymatic Properties of Metallo-{beta}-Lactamase VIM-5 from a Clinical Isolate of Enterobacter cloacae Antimicrob. Agents Chemother., October 1, 2005; 49(10): 4400 - 4403. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Poirel, L. Brinas, A. Verlinde, L. Ide, and P. Nordmann BEL-1, a Novel Clavulanic Acid-Inhibited Extended-Spectrum {beta}-Lactamase, and the Class 1 Integron In120 in Pseudomonas aeruginosa Antimicrob. Agents Chemother., September 1, 2005; 49(9): 3743 - 3748. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Pagani, C. Colinon, R. Migliavacca, M. Labonia, J.-D. Docquier, E. Nucleo, M. Spalla, M. Li Bergoli, and G. M. Rossolini Nosocomial Outbreak Caused by Multidrug-Resistant Pseudomonas aeruginosa Producing IMP-13 Metallo-{beta}-Lactamase J. Clin. Microbiol., August 1, 2005; 43(8): 3824 - 3828. [Abstract] [Full Text] [PDF]
|
|