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JAC Advance Access originally published online on December 8, 2004
Journal of Antimicrobial Chemotherapy 2005 55(1):115-118; doi:10.1093/jac/dkh500
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JAC vol.55 no.1 © The British Society for Antimicrobial Chemotherapy 2004; all rights reserved

A nosocomial outbreak of Acinetobacter baumannii isolates expressing the carbapenem-hydrolysing oxacillinase OXA-58

Claire Héritier1, Anne Dubouix2, Laurent Poirel1, Nicole Marty2 and Patrice Nordmann1,*

1 Service de Bactériologie-Virologie, Hôpital de Bicêtre, Assistance Publique/Hôpitaux de Paris, Faculté de Médecine Paris-Sud, Université Paris XI, 78 rue du Général Leclerc, 94275 Le Kremlin-Bicêtre cedex; 2 Laboratoire de Bactériologie-Hygiène, CHU Rangueil, 31403 Toulouse cedex 4, France

* Corresponding author. Tel: +33-1-45-21-36-32; Fax: +33-1-45-21-63-40; Email: nordmann.patrice{at}bct.ap-hop-paris.fr

Objective: The aim of this study was to analyse the spread of the blaOXA-58 gene as a source of carbapenem resistance in Acinetobacter baumannii in the burns unit of a university hospital in Toulouse in 2003–2004.

Methods: Six carbapenem-resistant A. baumannii isolates from six patients, and a carbapenem-resistant environmental A. baumannii isolate were collected in the burns unit of the Rangueil hospital (Toulouse). Susceptibility tests were carried out by disc diffusion and agar dilution methods. The detection of the blaOXA-58 gene was conducted by PCR followed by sequence analysis. Plasmids were extracted and hybridized with a probe specific for blaOXA-58. DNA fingerprints were obtained by pulsed-field gel electrophoresis of ApaI- or SmaI-digested chromosomal DNA of the tested strains.

Results: The multidrug-resistant clinical isolates had a similar 30 kb plasmid that encoded the carbapenem-hydrolysing ß-lactamase OXA-58. These isolates were clonally related. The unrelated environmental carbapenem-resistant A. baumannii isolate had a similar blaOXA-58-carrying plasmid, suggesting spread of this gene.

Conclusions: A novel oxacillinase was the source of carbapenem resistance in the A. baumannii isolates. Its gene was plasmid-, but not integron-borne.

Keywords: A. baumannii , carbapenem resistance , nosocomial infections


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