JAC Advance Access originally published online on October 7, 2004
Journal of Antimicrobial Chemotherapy 2004 54(5):936-939; doi:10.1093/jac/dkh457
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
JAC vol.54 no.5 © The British Society for Antimicrobial Chemotherapy 2004; all rights reserved
Nucleotide sequence and organization of the multiresistance plasmid pSCFS1 from Staphylococcus sciuri
1 Institut für Tierzucht, Bundesforschungsanstalt für Landwirtschaft (FAL), Höltystrasse 10, 31535 Neustadt-Mariensee, Germany; 2 Department of Pathobiology, University of Washington, Seattle, WA 98195, USA
* Corresponding author. Tel: +49-5034-871-241; Fax: +49-5034-871-246; Email: stefan.schwarz{at}fal.de
Objectives: The multiresistance plasmid pSCFS1 from Staphylococcus sciuri was sequenced completely and analysed with regard to its gene organization and the putative role of a novel ABC transporter in antimicrobial resistance.
Methods: Plasmid pSCFS1 was transformed into Staphylococcus aureus RN4220, overlapping restriction fragments were cloned into Escherichia coli plasmid vectors and sequenced. For further analysis of the ABC transporter, a 
3 kb EcoRV–HpaI fragment was cloned into the staphylococcal plasmid pT181MCS and the respective S. aureus RN4220 transformants were subjected to MIC determination.
Results: A total of 14 ORFs coding for proteins of >100 amino acids were detected within the 17 108 bp sequence of pSCFS1. Five of them showed similarity to recombination/mobilization genes while another two were similar to plasmid replication genes. In addition to the previously described genes cfr for chloramphenicol/florfenicol resistance and erm(33) for inducible resistance to macrolide-lincosamide-streptogramin B resistance, a Tn554-like spectinomycin resistance gene and Tn554-related transposase genes were identified. Moreover, a novel ABC transporter was detected and shown to mediate low-level lincosamide resistance.
Conclusion: Plasmid pSCFS1 is composed of various parts which show similarity to sequences known to occur on plasmids or transposons of Gram-positive, but also Gram-negative bacteria. It is likely that pSCFS1 represents the result of inter-plasmid recombination events also involving the truncation of a Tn554-like transposon.
Keywords: macrolide resistance , ABC transporter , low-level clindamycin resistance , florfenicol resistance , spectinomycin resistance
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  L. K. Smith and A. S. Mankin Transcriptional and Translational Control of the mlr Operon, Which Confers Resistance to Seven Classes of Protein Synthesis Inhibitors Antimicrob. Agents Chemother., May 1, 2008; 52(5): 1703 - 1712. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. A. Arias, M. Vallejo, J. Reyes, D. Panesso, J. Moreno, E. Castaneda, M. V. Villegas, B. E. Murray, and J. P. Quinn Clinical and Microbiological Aspects of Linezolid Resistance Mediated by the cfr Gene Encoding a 23S rRNA Methyltransferase J. Clin. Microbiol., March 1, 2008; 46(3): 892 - 896. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Kehrenberg, F. M. Aarestrup, and S. Schwarz IS21-558 Insertion Sequences Are Involved in the Mobility of the Multiresistance Gene cfr Antimicrob. Agents Chemother., February 1, 2007; 51(2): 483 - 487. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Novotna and J. Janata A New Evolutionary Variant of the Streptogramin A Resistance Protein, Vga(A)LC, from Staphylococcus haemolyticus with Shifted Substrate Specificity towards Lincosamides Antimicrob. Agents Chemother., December 1, 2006; 50(12): 4070 - 4076. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. S. Long, J. Poehlsgaard, C. Kehrenberg, S. Schwarz, and B. Vester The Cfr rRNA Methyltransferase Confers Resistance to Phenicols, Lincosamides, Oxazolidinones, Pleuromutilins, and Streptogramin A Antibiotics. Antimicrob. Agents Chemother., July 1, 2006; 50(7): 2500 - 2505. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Kehrenberg and S. Schwarz Distribution of Florfenicol Resistance Genes fexA and cfr among Chloramphenicol-Resistant Staphylococcus Isolates. Antimicrob. Agents Chemother., April 1, 2006; 50(4): 1156 - 1163. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Poole Efflux-mediated antimicrobial resistance J. Antimicrob. Chemother., July 1, 2005; 56(1): 20 - 51. [Abstract] [Full Text] [PDF]
|
|