JAC Advance Access originally published online on August 25, 2004
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Journal of Antimicrobial Chemotherapy 2004 54(4):704-710; doi:10.1093/jac/dkh380
JAC vol.54 no.4 © The British Society for Antimicrobial Chemotherapy 2004; all rights reserved
Effect of the lysophospholipid analogues edelfosine, ilmofosine and miltefosine against Leishmania amazonensis
Dept. de Ultra-estrutura e Biologia Celular, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, 21045–900, Rio de Janeiro-RJ, Brazil
* Corresponding author. Tel: +55-21-2598-4330; Fax: +55-21-260-4434; Email: solange{at}ioc.fiocruz.br
Objectives: Analysis of the effect of edelfosine, ilmofosine and miltefosine on Leishmania amazonensis and of potential targets of these lysophospholipid analogues.
Methods: Quantification and ultrastructural analysis of the effect of lysophospholipid analogues on promastigote forms and on infected peritoneal macrophages, and flow cytometry analysis of treated promastigotes labelled with propidium iodide and rhodamine 123 (Rh123).
Results: The lysophospholipid analogues presented potent antiproliferative activity with IC50/3 days of 1.9–3.4 µM for promastigotes and 4.2–9.0 µM for intracellular amastigotes. Treatment with these analogues in Schneider medium for 1 day led to a dose-dependent decrease in Rh123 fluorescence, an effect more accentuated in edelfosine-treated parasites, suggesting interference with the potential of the mitochondrial membrane. In both forms of L. amazonensis, edelfosine induced extensive mitochondrial damage, multinucleation and, in promastigotes, also led to plasma membrane alterations, formation of autophagic structures and membranous arrangements inside the flagellar pocket.
Conclusions: The alkylglycerophosphocholines edelfosine and ilmofosine were more active than the alkylphosphocholine miltefosine against promastigotes and intracellular amastigotes of L. amazonensis, and ultrastructural and flow cytometry data indicate the mitochondrion as a target of edelfosine.
Keywords: L. amazonensis , alkylglycerophosphocholines , flow cytometry , chemotherapy , electron microscopy
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