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JAC Advance Access originally published online on August 12, 2004
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Journal of Antimicrobial Chemotherapy 2004 54(3):680-683; doi:10.1093/jac/dkh391
JAC vol.54 no.3 © The British Society for Antimicrobial Chemotherapy 2004; all rights reserved.

Enterococcus faecium N03-0072 carries a new VanD-type vancomycin resistance determinant: characterization of the VanD5 operon

David A. Boyd1, Pamela Kibsey2, Diane Roscoe3 and Michael R. Mulvey1,* on behalf of the Canadian Nosocomial Infection Surveillance Program (CNISP){dagger}

1 Nosocomial Infections, National Microbiology Laboratory, Health Canada, 1015 Arlington Street, Winnipeg, Manitoba, R3E 3R2; 2 Victoria General Hospital, Victoria, British Columbia; 3 Vancouver General Hospital, Vancouver, British Columbia, Canada

* Corresponding author. Tel: +1-204-789-2133; Fax: +1-204-789-5020; Email: michael_mulvey{at}hc-sc.gc.ca

Objectives: To genotypically characterize the vancomycin resistance mechanism of Enterococcus faecium N03-0072, which was negative by PCR for the currently known van genotypes.

Methods: PCR was used to amplify the entire vancomycin resistance operon and the complete nucleotide sequence was determined by dideoxy cycle sequencing.

Results: Analysis revealed a VanD-type operon with 94% nucleotide identity to the VanD4 operon and 90% nucleotide identity to the VanD1/D3 operons. A set of universal primers was designed in order to identify all current vanD variants by PCR.

Conclusions: E. faecium N03-0072 carries a new VanD-type operon, designated VanD5.

Keywords: glycopeptide resistance , van PCR , E. faecium


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