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JAC Advance Access originally published online on May 18, 2004
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Journal of Antimicrobial Chemotherapy 2004 54(1):122-129; doi:10.1093/jac/dkh271
JAC vol.54 no.1 © The British Society for Antimicrobial Chemotherapy 2004; all rights reserved.

Molecular characterization of penicillin non-susceptible Streptococcus pneumoniae in Christchurch, New Zealand

David C. Bean1,*, Rosemary B. Ikram2 and John D. Klena3

1 Centre for Infectious Disease, Barts and The London School of Medicine and Dentistry, Queen Mary College, University of London, London E1 2AD, UK; 2 Medlab South Ltd., Christchurch, New Zealand; 3 School of Molecular Biosciences, Washington State University, Pullman, Washington, USA

* Corresponding author. Tel: +44-20-7377-7000, extension 3228; Fax: +44-20-7377-7259; Email: d.c.bean{at}qmul.ac.uk

Objectives: To determine the epidemiological relationship between non-invasive penicillin non-susceptible Streptococcus pneumoniae isolates collected in the Christchurch community between 1997 and 2001.

Methods: One hundred and ninety-seven pneumococcal isolates were examined by macrorestriction profile analysis of SmaI-digested genomic DNA separated by PFGE and restriction fragment length polymorphism analysis of penicillin binding protein genes.

Results: Four major clonal lineages were identified, the largest and most homogenous containing 95 (48.2%) of the isolates, the bulk of which (93.7%), had identical macrorestriction patterns. Members of this clonal group were multidrug-resistant and exhibited high resistance to third-generation cephalosporins, with MICs ≥8.0 mg/L not uncommon (23.1%). Two of the clonal groups, each containing 24 (12.2%) isolates, appeared indistinguishable from the globally widespread Spain23F-1 and France9V-3 strains, respectively. The fourth (12.7% of isolates) multidrug-resistant clone possessed intermediate penicillin susceptibility (MIC 0.12 mg/L).

Conclusions: This study shows that several distinct penicillin-resistant pneumococcal clones are present in the Christchurch community, most of which appear to have been imported into New Zealand.

Keywords: pneumococci , PFGE , cephalosporins , epidemiology


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D. C. Bean and J. D. Klena
Characterization of major clones of antibiotic-resistant Streptococcus pneumoniae in New Zealand by multilocus sequence typing
J. Antimicrob. Chemother., March 1, 2005; 55(3): 375 - 378.
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