JAC Advance Access originally published online on April 29, 2004
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Journal of Antimicrobial Chemotherapy (2004) 53, 947-951
© 2004 The British Society for Antimicrobial Chemotherapy
New aminoglycoside acetyltransferase gene, aac(3)-Id, in a class 1 integron from a multiresistant strain of Vibrio fluvialis isolated from an infant aged 6 months
1 Graduate School of Biosphere Science, Hiroshima University, Higashi-Hiroshima; 2 Graduate School of Natural Science and Technology, Okayama University, Okayama; 3 Department of Bacteriology, National Institute of Infectious Diseases, Tokyo, Japan; 4 National Institute of Cholera and Enteric Diseases, Calcutta, India
Received 23 December 2003; returned 2 February 2004; revised 2 March 2004; accepted 3 March 2004
Objectives: To characterize the molecular basis of antibiotic resistance in a multidrug-resistant clinical isolate of Vibrio fluvialis H-08942.
Patient and methods: V. fluvialis H-08942 was isolated from a hospitalized infant aged 6 months suffering from cholera-like diarrhoea in India in 2002. The broth microdilution method was used to determine the MICs of a range of antibiotics for this strain. PCR, DNA sequencing, Southern hybridization, cloning and expression were used to characterize the molecular basis of antibiotic resistances.
Results: V. fluvialis H-08942 showed resistance to chloramphenicol, streptomycin, spectinomycin, co-trimoxazole, ampicillin, furazolidone, nalidixic acid and gentamicin. A class 1 integron that contains a novel aminoglycoside acetyltransferase gene, aac(3)-Id, and aminoglycoside adenyltransferase gene, aadA7, was characterized. The aac(3)-Id gene product was found to share 50%, 45% and 44% identity to AAC(3)-Ic, AAC(3)-Ia, and AAC(3)-Ib, respectively. Both aac(3)-Id and aadA7 genes were cloned and expressed in Escherichia coli. Phylogenetic analysis suggested that the aac(3)-Id represents a fourth evolutionary lineage in the aminoglycoside acetyltransferase genes. Southern hybridization showed that this integron is located in the chromosome.
Conclusions: In this study we identified a new type of aminoglycoside acetyltransferase gene, aac(3)-Id. In addition, this is the first report of identification of antibiotic resistance genes and a class 1 integron in V. fluvialis.
Keywords: gentamicin resistance, aminoglycoside adenyltransferase, aadA7, cholera-like diarrhoea
* Corresponding author. Tel/Fax: +81-824-24-7897; E-mail: tadashis{at}hiroshima-u.ac.jp
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