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JAC Advance Access originally published online on March 31, 2004
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Journal of Antimicrobial Chemotherapy (2004) 53, 739-742
© 2004 The British Society for Antimicrobial Chemotherapy

A comparative study of the disc diffusion method with the broth microdilution and Etest methods for voriconazole susceptibility testing of Aspergillus spp.

M. C. Serrano1, M. Ramírez1, D. Morilla1, A. Valverde1, M. Chávez1, A. Espinel-Ingroff2, R. Claro, A. Fernández3, C. Almeida3 and E. Martín-Mazuelos1,*

1 Servicio de Microbiología, Hospital Universitario de Valme, Ctra Cádiz s/n, Sevilla 41014; 3 Servicio de Bioestadística, Unidad Investigación, Hospital Universitario de Valme, Sevilla, Spain; 2 Division of Infectious Diseases, Medical College of Virginia, Virginia Commonwealth University, Richmond, VA 2329-80049, USA

Received 3 November 2003; returned 23 December 2003; revised 28 January 2004; accepted 8 February 2004

Objective: The activity of voriconazole against Aspergillus spp. (n = 77) was tested by the Etest, disc diffusion and the NCCLS M38-A methods.

Methods: Four Rhizomucor spp. isolates were included to study the suitability of the three susceptibility testing methods to detect isolates resistant to voriconazole. The disc diffusion method performed on Mueller–Hinton agar (Difco) supplemented with 2% glucose and Methylene Blue (0.5 mg/L) gave zone diameters with very clear border edges.

Results: The disc diffusion method showed excellent correlation with the Etest and the NCCLS methods.

Conclusion: On the basis of our results, it appears that the disc diffusion test is a useful method for testing the activity of voriconazole against Aspergillus spp.

Keywords: antifungals, susceptibility tests

* Corresponding author. Tel: +34-9550-15481/80; Fax: +34-9550-15481; E-mail: estrella.martin.sspa{at}juntadeandalucia.es


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