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JAC Advance Access originally published online on November 12, 2003
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Journal of Antimicrobial Chemotherapy (2003) 52, 1015-1017
© 2003 The British Society for Antimicrobial Chemotherapy

SHV-34: an extended-spectrum ß-lactamase encoded by an epidemic plasmid

John Heritage1,*, Philip A. Chambers1, Caroline Tyndall2 and E. Stephen Buescher2

1 Division of Microbiology, School of Biochemistry and Molecular Biology, University of Leeds, Leeds LS2 9JT, UK; 2 Center for Pediatric Research, Department of Pediatrics, Eastern Virginia Medical School, Children’s Hospital of The King’s Daughters, 855 West Brambleton Avenue, Norfolk, Virginia 23510–1001, USA

Received 21 May 2003; returned 3 July 2003, revised 6 October 2003; accepted 9 October 2003

Objectives: To elucidate the causes for treatment failure in children given extended-spectrum cephalosporins.

Methods: During April 1998–March 2000, 18 isolates of members of the family Enterobacteriaceae, fulfilling microbiological criteria for carriage of extended-spectrum ß-lactamases (ESBLs) and carrying blaSHV, were isolated from paediatric inpatients. The collection was subjected to a retrospective molecular analysis.

Results: Three species were represented in the collection: Citrobacter koseri (one isolate), Escherichia coli (one isolate) and Klebsiella pneumoniae (16 isolates). A common plasmid was found in these bacteria, as judged by restriction endonuclease digestion. This was able to transfer an ESBL phenotype from donors to a laboratory strain of E. coli. Nucleotide sequence analysis revealed that this phenotype was associated with a new variant in blaSHV encoding SHV-34.

Conclusions: Analysis reveals the presence of an epidemic plasmid in this collection of bacteria. This carries a gene encoding the SHV-34 ESBL, described for the first time in this report. Nucleotide sequence analysis shows that there is a mutation from A->G affecting the codon at amino acid position 64 (GAA->GGA), changing the glutamic acid typically seen in this position to glycine.

Keywords: ESBLs, Enterobacteriaceae, SHV ß-lactamase

* Corresponding author. Tel: +44-113-343-5592; Fax: +44-113-343-5638; E-mail: j.heritage{at}leeds.ac.uk


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