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JAC Advance Access originally published online on September 30, 2003
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Journal of Antimicrobial Chemotherapy (2003) 52, 796-800
© 2003 The British Society for Antimicrobial Chemotherapy

Development of a microdilution method to evaluate Mycobacterium tuberculosis drug susceptibility

Elena Banfi*, Giuditta Scialino and Carlo Monti-Bragadin

Dipartimento di Scienze Biomediche, sez. Microbiologia, Università degli Studi di Trieste, Trieste, Italy

Received 30 July 2002; returned 6 February 2003; revised 21 February 2003; accepted 9 August 2003

A new rapid microdilution method, employing the dye resazurin as an indicator of mycobacterial growth, was developed to evaluate drug susceptibility of Mycobacterium tuberculosis reference strain H37Rv and of 13 M. tuberculosis susceptible or multidrug-resistant clinical strains. Different growth conditions were evaluated. The MICs of isoniazid, rifampicin, streptomycin and ethambutol were determined by the Microdilution Resazurin Assay (MRA) and the results compared with those obtained by the agar proportion method; complete agreement was always obtained. MRA resulted in a rapid, reliable, simple and inexpensive coloured method suitable for testing the susceptibility of M. tuberculosis clinical strains to first-line drugs; its employment in evaluating new antibacterial molecules is also suggested.

Keywords: M. tuberculosis, resazurin, MIC evaluation, microdilution assay, first-line antitubercular drugs

* Corresponding author. Tel: +39-40-5587187; Fax: +39-40-351668; E-mail: banfi{at}dsbmail.units.it


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