JAC Advance Access originally published online on September 1, 2003
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Journal of Antimicrobial Chemotherapy (2003) 52, 583-590
© 2003 The British Society for Antimicrobial Chemotherapy
Genetic characterization of a novel metallo-ß-lactamase gene, blaIMP-13, harboured by a novel Tn5051-type transposon disseminating carbapenemase genes in Europe: report from the SENTRY worldwide antimicrobial surveillance programme
1 Department of Pathology and Microbiology, University of Bristol, Bristol BS8 1TD, UK; 2 The JONES Group/JMI Laboratories, North Liberty, Iowa; 3 Tufts University School of Medicine, Boston, Massachusetts, USA
Received 23 May 2003, returned 13 June 2003, revised 8 July 2003; accepted 9 July 2003
Objective: In 2001, as part of the SENTRY worldwide antimicrobial surveillance programme, Pseudomonas aeruginosa 86–14571A was isolated at a hospital in Rome from a cancer patient with a bloodstream infection. The isolate was resistant to all antibiotics except amikacin, and displayed an imipenem MIC of 64 mg/L that decreased to 8 mg/L in the presence of EDTA. The resistance determinant was investigated.
Methods: The resistant determinant was cloned in Escherichia coli using a shotgun cloning approach.
Results: Sequence analysis revealed the presence of a novel IMP-type metallo-ß-lactamase (MBL) gene, blaIMP-13. This encoded a protein displaying most identity to IMP variants: 93% and 92.3% identity, respectively, to IMP-8 and IMP-2 (previously identified in Italy). The protein had 19 amino acid changes from IMP-2 and 17 amino acid changes from IMP-8. The blaIMP-13 gene was found as a gene cassette in the first position of a class 1 integron. A 25 bp inverted repeat sequence IRi was identified 174 bp upstream of the class I integrase, which suggests that the integron is found on a Tn402-like transposon, or defective transposon derivative. This element, in turn, is located in the transposition locus (tnp region) of a Tn21 subfamily transposon that showed most identity to Tn5051, a transposon recently identified from a strain of Pseudomonas putida isolated in New York. Interestingly, the insertion point of the Tn402-like transposon and the sequence of the Tn5051-like genes were identical to those of the genetic element harbouring blaVIM-2 recently identified in Poland.
Conclusions: The resistance determinant of P. aeruginosa 86–14571A is a novel IMP-type MBL carried on a composite transposon responsible for wide geographical dissemination of MBL genes in Europe.
Keywords: metallo-ß-lactamases, Pseudomonas aeruginosa, integron Tn21, Tn402
* Corresponding author. Tel: +44-117-9287522; Fax: +44-117-9287896; E-mail: Mark.Toleman{at}bristol.ac.uk
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