JAC Advance Access originally published online on July 15, 2003
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Journal of Antimicrobial Chemotherapy (2003) 52, 300-302
© 2003 The British Society for Antimicrobial Chemotherapy
Tolerance to vancomycin in a multiresistant, Colombian isolate of Streptococcus pneumoniae
1 Microbiology Group, Instituto Nacional de Salud, Avenida Calle 26 No 51–60, Bogotá; 2 Bacterial Molecular Genetics Unit, Centro de Investigaciones, Universidad El Bosque, Bogotá, D.C., Colombia
Received 20 January 2003; returned 5 May 2003; revised 5 May 2003; accepted 15 May 2003
The present study was set up to determine vancomycin tolerance in Colombian, multiresistant, invasive, paediatric isolates of Streptococcus pneumoniae. A total of seven clinical isolates of penicillin-resistant S. pneumoniae, serotype 14, corresponding to the Spain9v-3 clone, were studied. Isolates were grown in Todd Hewitt broth at 37°C. Growth (A595) and cell viability (cfu/mL) were monitored hourly for 4 h after addition of vancomycin at 10x MIC. S. pneumoniae R6 was used as a control for growth. For R6, the mean decrease in A595 and cell viability (log10 cfu/mL) after 4 h of vancomycin treatment (in 10 independent experiments) was 95.1% and 4.2 log10, respectively. Six out of seven isolates exhibited similar behaviour. In contrast, for S. pneumoniae E239, the mean decrease in A595 and cell viability was 48.5% and 2.2 log10 after 4 h of vancomycin exposure, which suggested the presence of tolerance to vancomycin. The emergence of vancomycin-tolerant strains is of great concern in Colombia, owing to the alarming increase in resistance to penicillin.
Keywords: antimicrobial tolerance, vancomycin, Colombian isolates, killing curves, Streptococcus pneumoniae
* Corresponding author. Tel: +571-2227700 ext 446; Fax: +571-2221093; E-mail: mhidalgo{at}ins.gov.co
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