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JAC Advance Access originally published online on May 29, 2003
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Journal of Antimicrobial Chemotherapy (2003) 52, 135-137
© 2003 The British Society for Antimicrobial Chemotherapy

Determination by HPLC of chlortetracycline in pig faeces

Julie Sunderland1,*, Andrew M. Lovering1, Caroline M. Tobin1, Alasdair P. MacGowan1, John M. Roe2 and Anne A. Delsol2

1 Bristol Centre for Antimicrobial Research and Evaluation, North Bristol NHS Trust, Southmead Hospital, Bristol BS10 5NB; 2 Division of Animal Health and Husbandry, Department of Clinical Veterinary Science, University of Bristol, Langford BS40 5DU, UK

Received 19 November 2002; returned 18 January 2003; revised 25 March 2003; accepted 6 April 2003

An HPLC assay used to determine chlortetracycline (CTC) in pig faeces is reported. Prodigy ODS3 (4.6 x 150 mm) was used for the stationary phase, whereas the mobile phase comprised oxalic acid, sodium oxalate and sodium decane sulfonate (66%)—each of 4 mM, and 34% acetonitrile. The mobile phase was pumped at a flow rate of 1 mL/min. Detection of CTC was by ultraviolet absorbance at 370 nm, and a 20 µL injection volume was used. Recovery from faeces was >90%, and coefficients of variability between runs were <10%. The lowest limit of quantification was 3.5 mg/kg, with an accuracy of <7% error. There was no interference from endogenous materials in the pig faeces, or commonly used antibiotics, and the method is suitable for use in drug disposition studies.

Keywords: chlortetracycline, HPLC, pig faeces

* Corresponding author. Tel: +44-117-959-5654; E-mail: sunderland_j@southmead.swest.nhs.uk


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