JAC Advance Access originally published online on June 12, 2003
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Journal of Antimicrobial Chemotherapy (2003) 52, 110-112
© 2003 The British Society for Antimicrobial Chemotherapy
Macrolide resistance determinants of group A streptococci in Ankara, Turkey
1 Microbiology and Clinical Microbiology Department of Fatih University School of Medicine, Ankara; 2 METIS Biotechnology Ltd, Ankara, Turkey
Received 4 January 2003; returned 28 January 2003; revised 11 February 2003; accepted 23 April 2003
Objectives: Macrolide–lincosamide–streptogramin B (MLSB) resistance determinants of group A streptococci (GAS) in Ankara, Turkey, were defined for the first time.
Isolates and methods: A total of 1355 GAS isolates, collected from three different regions of Ankara, were screened for erythromycin resistance. Resistance phenotypes were determined by a triple-disc test, and the gene determinants responsible were determined by PCR. MICs of erythromycin, clindamycin and spiramycin were measured for the resistant isolates, and susceptibility rates to some further antibiotics were determined.
Results: Thirty-six isolates (2.6%) were resistant to erythromycin. Of these, 17 (47.2%) expressed macrolide-restricted resistance (M phenotype), while the remainder expressed inducible (16 isolates, 44.4%) or constitutive (three isolates, 8.3%) MLSB resistance. All isolates of the M phenotype harboured the mef(A) gene. Of non-M isolates, 14 harboured erm(A) subclass erm(TR) and five had erm(B) genes. There was a significant relationship between tetracycline resistance and the inducible phenotype (P < 0.05). Macrolide resistance was significantly higher in adults (P < 0.05), and increased more than two-fold in 2002 compared with 2001 (P < 0.05).
Conclusion: The prevalence of macrolide resistance in GAS is low in Ankara; therefore, routine antimicrobial susceptibility testing against these agents seems unwarranted.
Keywords: Streptococcus pyogenes, macrolide–lincosamide–streptogramin B resistance, MLSB
* Correspondence address. Fatih Üniversitesi Hastanesi, Merkez Laboratuvar, Alpaslan Türke_ Cad. No: 57, 06510 Emek, Ankara, Turkey. Tel: +90-312-212-62-62; Fax: +90-312-215-36-14; E-mail: zcacikgoz{at}yahoo.com
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