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JAC Advance Access originally published online on February 25, 2003
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Journal of Antimicrobial Chemotherapy (2003) 51, 843-847
© 2003 The British Society for Antimicrobial Chemotherapy

In vitro effect on Cryptosporidium parvum of short-term exposure to cathelicidin peptides

Andrea Giacometti1,*, Oscar Cirioni1, Maria Simona Del Prete1, Barbara Skerlavaj2, Raffaella Circo2, Margherita Zanetti2,3 and Giorgio Scalise1

1 Institute of Infectious Diseases and Public Health, University of Ancona; 2 Department of Biomedical Sciences and Technology, University of Udine; 3 National Laboratory CIB, Area Science Park, Padriciano 99, 34012 Trieste, Italy

Received 7 June 2002; returned 26 June 2002; revised 22 October 2002; accepted 13 January 2003

Two laboratory methods, a cell culture system and double fluorogenic staining, were used to study the viability and infective ability of Cryptosporidium parvum sporozoites and oocysts after short-term exposure to four cathelicidin peptides. The compounds, SMAP-29, BMAP-28, PG-1 and Bac7(1-35), exerted a strong cytotoxic effect on sporozoites, but did not affect the viability and function of oocysts consistently. Overall, in the sporozoite series, a percentage of the viable population decreased rapidly to less than detectable levels after 15 and 60 min exposure to the peptides at concentrations of 100 and 10 µg/mL, respectively. In the oocyst series, no compound produced complete inhibition of parasite growth: 60–85% of the oocyst population was viable after 180 min exposure at 100 µg/mL. SMAP-29 exerted the highest activity against both sporozoites and oocysts.

Keywords: cathelicidins, Cryptosporidium parvum, susceptibility

* Correspondence address. Clinica Malattie Infettive, c/o Ospedale Regionale, Via Conca, I-60020 Ancona, Italy. Tel: +39-071-596-3715; Fax: +39-071-596-3468; E-mail: anconacmi{at}interfree.it


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