JAC Advance Access originally published online on February 11, 2003
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Journal of Antimicrobial Chemotherapy (2003) 51, 631-638
© 2003 The British Society for Antimicrobial Chemotherapy
Production of CTX-M-3 extended-spectrum ß-lactamase and IMP-1 metallo ß-lactamase by five Gram-negative bacilli: survey of clinical isolates from seven laboratories collected in 1998 and 2000, in the Kinki region of Japan
1 Division of Clinical Microbiology, Department of Clinical Laboratory, Wakayama Rosai Hospital, 435 Koya, Wakayama; 2 Department of Clinical Pathology, Tenri Hospital, 200 Misima, Tenri, Nara 632-8552; 3 Bacteriological Testing Section of Central Laboratory, FALCO Biosystems Ltd, Central Laboratory, Kyoto; 4 Department of Clinical and Laboratory Medicine, National Cardiovascular Center, Osaka; 5 Department of Clinical Laboratory, Shiga Medical Center for Adults, Shiga; 6 Division of Clinical Microbiology, Department of Medical Technology, Kinki University School of Medicine, Osaka; 7 Department of Clinical Laboratory, Kobe University Hospital, Hyogo, Japan
Received 3 April 2002; returned 19 August 2002; revised 4 November 2002; accepted 25 November 2002
The aim of this study was to research the distribution in the Kinki region of Japan of Enterobacteriaceae and Pseudomonas aeruginosa that produce extended-spectrum ß-lactamase (ESBL) and metallo ß-lactamase (MBL). One thousand isolates, 200 of each of four enterobacterial species (i.e. Escherichia coli, Klebsiella pneumoniae, Enterobacter cloacae and Serratia marcescens) and 200 of P. aeruginosa, were collected from seven different laboratories during two 2 month periods, one in 1998 and one in 2000. A double-disc synergy test (DDST) and 2-mercaptopropionic acid inhibition test (2-MPAT) were used to confirm ß-lactamase-producing isolates. The DDST was positive for one isolate of E. coli, five of K. pneumoniae, two of E. cloacae and 14 of S. marcescens. The 2-MPAT was positive for five isolates of S. marcescens and two of P. aeruginosa. We identified the ß-lactamase type of each isolate by molecular confirmatory tests (isoelectric focusing, PCR and DNA sequencing): CTX-M-3 ESBLs (three isolates of K. pneumoniae, two of E. cloacae and 13 of S. marcescens), CTX-M-2 ESBL (one isolate of K. pneumoniae), SHV-12 ESBLs (one isolate of E. coli and one of S. marcescens), CTX-M-3 and SHV-12 combination ESBL (one isolate of K. pneumoniae) and IMP-1 MBLs (five isolates of S. marcescens and two of P. aeruginosa). In conclusion, many species of Gram-negative bacilli that produce CTX-M-3 ESBLs and IMP-1 MBLs were disseminated widely in different hospitals of the Kinki region of Japan. Therefore, monitoring of laboratory bacterial ecology seems important to stop the spread of these strains through nosocomial outbreaks.
* Corresponding author. Tel: +81-743-63-5611, ext. 8665; Fax: +81-743-62-5576; E-mail: microbiology{at}tenriyorozu-hp.or.jp
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