JAC Advance Access originally published online on January 6, 2003
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Journal of Antimicrobial Chemotherapy (2003) 51, 397-400
© 2003 The British Society for Antimicrobial Chemotherapy
Characterization of a new integron containing VIM-2, a metallo- ß-lactamase gene cassette, in a clinical isolate of Enterobacter cloacae
1 Departments of Clinical Pathology, 7 Internal Medicine and 8 Therapeutic Radiology, Kosin University College of Medicine, Amnam-Dong 34, Seo-Ku, Busan; 2 Research Institute of Bacterial Resistance and 3 Department of Clinical Pathology, Yonsei University College of Medicine, Seoul; 4 Department of Genetic Engineering, Youngdong University, Youngdong; 5 SJ Hightech, Busan; 6 Department of Clinical Pathology, Dong-A University College of Medicine, Busan, South Korea
Received 7 March 2002; returned 19 June 2002; revised 14 October 2002; accepted 18 October 2002
We report the first description of a new integron containing blaVIM-2, a metallo-ß-lactamase gene from Enterobacter cloacae KU680, which was isolated from peritoneal fluid of a liver cirrhosis patient in South Korea. Antibiotic susceptibility testing, and modified Hodge and EDTA-disc synergy tests, were carried out to screen for metallo-ß-lactamase-producing strains. PCR and sequence analysis were used to identify and analyse the blaVIM-2-containing integron. The isolate was resistant to most ß-lactams, including imipenem, and demonstrated a positive modified Hodge and EDTA-disc synergy test, which are findings suggesting a metallo-ß-lactamase. Preliminary PCR-based experiments detected the metallo-ß-lactamase gene blaVIM-2. Sequencing of the 4392 bp cloned PCR amplicon, containing the gene cassette blaVIM-2, revealed the structure of the class 1 integron. The integron also contained additional insert gene cassettes, aadA, and unknown open reading frames ‘orfII’ and ‘orfIII’. To the best of our knowledge, this is the first time that this metallo-ß-lactamase gene has been detected in E. cloacae.
* Corresponding author. Tel: +82-51-990-6373; Fax: +82-51-990-3034; E-mail: kscpjsh{at}ns.kosinmed.or.kr
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