Skip Navigation


JAC Advance Access originally published online on January 6, 2003
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
51/2/275    most recent
dkg054v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (3)
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Jonas, D.
Right arrow Articles by Daschner, F. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Jonas, D.
Right arrow Articles by Daschner, F. D.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?


Journal of Antimicrobial Chemotherapy (2003) 51, 275-280
© 2003 The British Society for Antimicrobial Chemotherapy

Development and mechanism of fluoroquinolone resistance in Legionella pneumophila

Daniel Jonas1,*, Inge Engels1, Doris Hartung1, Jan Beyersmann2, Uwe Frank1 and Franz D. Daschner1

1 Institute of Environmental Medicine and Hospital of Epidemiology, University Hospital of Freiburg, Hugstetter Strasse 55, D-79106 Freiburg; 2 Freiburg Centre for Data Analysis and Modelling, University of Freiburg and Institute of Medical Biometry and Medical Informatics, University Hospital of Freiburg, Freiburg, Germany

Received 22 July 2002; returned 8 October 2002; revised 17 October 2002; accepted 22 October 2002

The potential for selection in vitro of Legionella pneumophila mutants resistant to fluoroquinolones was investigated. Six distinct clinical isolates of L. pneumophila were subcultured in subinhibitory concentrations of ciprofloxacin, levofloxacin, clinafloxacin, trovafloxacin and moxifloxacin until MICs increased at least eight-fold. The numbers of serial passages required in microbroth dilution series were determined. The gyrA gene of the six parental strains, and 12 selected mutant strains, was sequenced. The five quinolones differed markedly in their ability to select mutants with decreased susceptibility. The average number of serial passages required was low in the cases of clinafloxacin (n = 10.6), ciprofloxacin and levofloxacin (both n = 13), but notably higher for trovafloxacin (n = 26.6) and moxifloxacin (n = 22.5). Five mutants treated with ciprofloxacin and three treated with moxifloxacin showed Thr83->Lys or Thr83->Ile amino acid changes in the gyrA gene. In conclusion, different quinolones lose their antimicrobial effect after a varying number of passages. This study demonstrated, for the first time to our knowledge, that gyrA in L. pneumophila is a possible target of fluoroquinolones.

Keywords: fluoroquinolone resistance, gyrA, Legionella pneumophila

* Corresponding author. Tel: +49-761-270-5445; Fax: +49-761-270-5485; E-mail: djonas{at}IUK3.UKL.uni-Freiburg.de


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J Antimicrob ChemotherHome page
I. Almahmoud, E. Kay, D. Schneider, and M. Maurin
Mutational paths towards increased fluoroquinolone resistance in Legionella pneumophila
J. Antimicrob. Chemother., August 1, 2009; 64(2): 284 - 293.
[Abstract] [Full Text] [PDF]

Home page
 J Antimicrob ChemotherHome page
N. Roch and M. Maurin
Antibiotic susceptibilities of Legionella pneumophila strain Paris in THP-1 cells as determined by real-time PCR assay
J. Antimicrob. Chemother., June 1, 2005; 55(6): 866 - 871.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.