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JAC Advance Access originally published online on November 28, 2002
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Journal of Antimicrobial Chemotherapy (2003) 51, 135-139
© 2003 The British Society for Antimicrobial Chemotherapy

Comparison of levels of HIV-1 resistance to protease inhibitors by recombinant versus conventional virus phenotypic assay and two genotypic interpretation procedures in treatment-naive and HAART-experienced HIV-infected patients

Stefania Paolucci1, Fausto Baldanti1,2, Maurizio Zavattoni1, Giuditta Comolli1, Nazzarena Labò1, Stefano Menzo3, Massimo Clementi4 and Giuseppe Gerna1,*

1 Servizio di Virologia and 2 Laboratori Sperimentali di Ricerca, IRCCS Policlinico San Matteo, 27100 Pavia; 3 Istituto di Microbiologia, Università di Ancona, Ancona; 4 Università Vita-Salute San Raffaele, IRCCS San Raffaele, Milan, Italy

Received 15 March 2002; returned 22 July 2002; revised 12 September 2002; accepted 27 September 2002

Objectives: To compare genotypic and phenotypic HIV-1 drug resistance assays.

Methods: Protease inhibitor (PI) susceptibility was phenotypically analysed in HIV-1 isolates and recombinant viruses expressing proteases from viral isolates. Two genotypic interpretation methods were carried out in parallel.

Results: Entirely concordant resistance levels were shown in 5/10 (50%) highly active anti-retroviral therapy (HAART)-experienced patients, whereas minor discrepancies were observed in the remaining five patients.

Conclusions: The four assays provide comparable results. The recombinant virus phenotypic assay may provide the most accurate evaluation of resistance; however, genotypic interpretation procedures are helpful for daily therapeutic decisions.

* Corresponding author. Tel: +39-0382-502420; Fax: +39-0382-502599; E-mail: g.gerna{at}smatteo.pv.it


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