JAC Advance Access originally published online on October 22, 2002
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Journal of Antimicrobial Chemotherapy (2002) 50, 723-726
© 2002 The British Society for Antimicrobial Chemotherapy
Characterization of a laboratory-generated variant of BPS ß-lactamase from Burkholderia pseudomallei that hydrolyses ceftazidime
1 Division of Infectious Diseases, Department of Microbiology and 2 HKU-Pasteur Research Centre, Faculty of Medicine, The University of Hong Kong, Queen Mary Hospital, Pokfulam Road, Pokfulam, Hong Kong SAR, China
Received 11 April 2002; returned 3 July 2002; revised 14 August 2002; accepted 20 August 2002
Burkholderia pseudomallei produces an Ambler class A ß-lactamase, known as BPS-1. The ß-lactamase gene from a laboratory-derived, ceftazidime-resistant strain of B. pseudomallei (LH-1-2) was cloned and expressed in Escherichia coli. The ß-lactamase, named BPS-1m, had an identical isoelectric focusing point (pI 7.7) to that of BPS-1, but differed in having a stronger hydrolytic activity against ceftazidime. Susceptibility testing showed that BPS-1m when expressed in E. coli conferred resistance to ceftazidime (MIC 
32 mg/L). The amino acid sequence of BPS-1m differed from that of BPS-1 by a Pro-to-Ser change at position 167 in the omega loop.
* Corresponding author. Tel: +852-2855-4897; Fax: +852–2855-1241; E-mail: plho{at}hkucc.hku.hk
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