JAC Advance Access originally published online on September 20, 2002
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Journal of Antimicrobial Chemotherapy (2002) 50, 719-722
© 2002 The British Society for Antimicrobial Chemotherapy
Comparative evaluation of two different methods of inoculum preparation for antifungal susceptibility testing of filamentous fungi
Servicio de Micología, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Ctra Majadahonda-Pozuelo Km 2, 28220 Majadahonda, Madrid, Spain
Received 5 April 2002; returned 18 June 2002; revised 11 July 2002; accepted 26 July 2002
Two different methods of inoculum preparation for susceptibility testing were analysed. The first method was adjustment of inoculum size by haemocytometer counting. The second method was spectrophotometric adjustment at 530 nm. The reliability of both methods was assessed by colony counting. The overall agreement between colony counting and haemocytometer counts was 93.6%, and the intraclass coefficient correlation was 0.71 (P < 0.05). Pearson’s correlation index between colony counts and optical density values was –0.059 (P > 0.05). Optical densities ranged between 0.01 and 1.2, showing less reproducibility than expected by the NCCLS M38-P standard. Haemocytometer counting is a more reliable method of inoculum preparation for antifungal susceptibility testing.
* Corresponding author. Tel: +34-91-5097961; Fax: +34-91-5097966; E-mail: juanl.rodriguez-tudela{at}isciii.es
The Eurofung Network participants are listed in the Acknowledgements.
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