JAC Advance Access originally published online on October 22, 2002
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Journal of Antimicrobial Chemotherapy (2002) 50, 681-688
© 2002 The British Society for Antimicrobial Chemotherapy
Characterization of CMY-type ß-lactamases in clinical strains of Proteus mirabilis and Klebsiella pneumoniae isolated in four hospitals in the Paris area
1 Service de Bactériologie, Hôpital Saint Antoine,UFR Saint-Antoine, 184 rue du Faubourg Saint-Antoine, 75012 Paris; 2 Service de Bactériologie, Hôpital Tenon, UFR Saint-Antoine, Paris; 3 Service de Bactériologie Hôpital Lariboisière; 4 Service de Bactériologie, CHU Cochin, AP-HP, Paris, France
Received 13 November 2001; returned 20 March 2002; revised 1 May 2002; accepted 2 August 2002
We isolated five clinical strains (three Proteus mirabilis and two Klebsiella pneumoniae) with ß-lactam resistance phenotypes consistent with production of an AmpC-type ß-lactamase. The predicted amino acid sequences of the enzymes were typical of class C ß-lactamases. The enzymes were identified as CMY-2, CMY-4 and a new CMY-variant ß-lactamase, CMY-12. The AmpC ß-lactamases from the two K. pneumoniae isolates were found to be encoded on self-transferable plasmids. The genes encoding the AmpC-type ß-lactamase produced by the three P. mirabilis isolates were chromosomal. Four of the five clinical isolates were from patients transferred from Greece, Algeria and Egypt; one of the K. pneumoniae strains was recovered from a French patient. PFGE analysis and rep-PCR fingerprinting showed that the two P. mirabilis isolates from Greek patients were closely related.
* Corresponding author. Tel: +33-1-49-28-29-77; E-mail: dominique.decre{at}sat.ap-hop-paris.fr
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