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JAC Advance Access originally published online on September 6, 2002
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Journal of Antimicrobial Chemotherapy (2002) 50, 589-592
© 2002 The British Society for Antimicrobial Chemotherapy

Fluoroquinolone resistance in Mycoplasma gallisepticum: DNA gyrase as primary target of enrofloxacin and impact of mutations in topoisomerases on resistance level

A. K. Reinhardt, I. Kempf, M. Kobisch and A. V. Gautier-Bouchardon*

Agence Française de Sécurité Sanitaire des Aliments, Laboratoire d’Etudes et de Recherches Avicoles et Porcines, Unité de Mycoplasmologie-Bactériologie, BP 53, 22440 Ploufragan, France

Received 9 January 2002; returned 15 April 2002; revised 27 May 2002; accepted 20 June 2002

Resistant mutants of Mycoplasma gallisepticum were selected in vitro by passaging strains 10 times in increasing concentrations of enrofloxacin. The regions of gyrA/gyrB and parC/parE, encoding the quinolone resistance-determining regions (QRDRs) of DNA gyrase and DNA topoisomerase IV, respectively, of the mutants obtained during different passages were sequenced. Several mutations were found in the four fluoroquinolone targets. Substitution of Ser-83->Arg in GyrA and Ser-80->Leu or Trp in ParC QRDRs seem to have the greatest impact on resistance to fluoroquinolones. The results obtained also suggest that the preferential target of enrofloxacin in M. gallisepticum is DNA gyrase.

* Corresponding author. Tel: +33-2-96-01-62-86; Fax: +33-2-96-01-62-73; E-mail: a.bouchardon{at}ploufragan.afssa.fr


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