Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (9)
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Heym, B.
Right arrow Articles by Nicolas-Chanoine, M.-H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Heym, B.
Right arrow Articles by Nicolas-Chanoine, M.-H.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?


Journal of Antimicrobial Chemotherapy (2002) 50, 323-329
© 2002 The British Society for Antimicrobial Chemotherapy

Multilocus sequence typing (MLST) shows that the ‘Iberian’ clone of methicillin-resistant Staphylococcus aureus has spread to France and acquired reduced susceptibility to teicoplanin

B. Heym, M. Le Moal, L. Armand-Lefevre and M.-H. Nicolas-Chanoine*

Microbiology Department, Hôpital Ambroise Paré, AP-HP and Faculté de Médecine Paris-Ouest, Université Paris V, 9 avenue Charles de Gaulle, 92100 Boulogne-Billancourt, France

Received 2 January 2002; returned 25 March 2002; revised 9 May 2002; accepted 31 May 2002

Infection by methicillin-resistant Staphylococcus aureus (MRSA) presents a serious problem, as these organisms are resistant to a wide range of antibiotics. Moreover, MRSA with reduced susceptibility to glycopeptides (GISA) have emerged in recent years. In our hospital we were faced with an outbreak of GISA between January and March 2000, involving six patients who were free of MRSA when the GISA isolate emerged, and five of whom had not been given glycopeptides. The initial GISA isolate from the six patients was compared with six ‘historical’ multiple-resistant MRSA isolates (1996–1999), which had not been found to be GISA by the routinely used method. Antibiotic susceptibility was studied through the disc diffusion method, and MICs of glycopeptides were determined by Etest, agar and broth dilution techniques. Molecular strain typing was done by PFGE and multilocus sequence typing (MLST). All 12 isolates that belonged to the gentamicin-resistant group of MRSA were susceptible to vancomycin, but showed reduced susceptibility to teicoplanin through at least one MIC method. PFGE typing yielded five different but closely related profiles, and eight of the 12 clinical isolates were classified into a single PFGE type. MLST yielded an identical allelic profile for all 12 isolates, corresponding to the profile (3-3-1-12-4-4-16) of the ‘Iberian’ clone HPV107 of MRSA. MLST allowed us to confirm the presence of the ‘Iberian’ MRSA clone HPV107 with the same allelic profile in our hospital for several years. We can now note that strains of this clone have acquired reduced susceptibility to teicoplanin.

* Corresponding author. Tel: +33-1-49-09-55-40; Fax: +33-1-49-09-59-21; E-mail: marie-helene.nicolas-chanoine{at}apr.ap-hop-paris.fr


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 MicrobiologyHome page
A. Olvera, M. Cerda-Cuellar, and V. Aragon
Study of the population structure of Haemophilus parasuis by multilocus sequence typing
Microbiology, December 1, 2006; 152(12): 3683 - 3690.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Microbiol.Home page
H. Y. Cha, D. C. Moon, C. H. Choi, J. Y. Oh, Y. S. Jeong, Y. C. Lee, S. Y. Seol, D. T. Cho, H.-H. Chang, S.-W. Kim, et al.
Prevalence of the ST239 Clone of Methicillin-Resistant Staphylococcus aureus and Differences in Antimicrobial Susceptibilities of ST239 and ST5 Clones Identified in a Korean Hospital
J. Clin. Microbiol., August 1, 2005; 43(8): 3610 - 3614.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Microbiol.Home page
N. van der Mee-Marquet, A.-S. Domelier, N. Girard, R. Quentin, and the Bloodstream Infection Study Group of the Relai
Epidemiology and Typing of Staphylococcus aureus Strains Isolated from Bloodstream Infections
J. Clin. Microbiol., December 1, 2004; 42(12): 5650 - 5657.
[Abstract] [Full Text] [PDF]

Home page
 J Antimicrob ChemotherHome page
P.-Y. Donnio, L. Preney, A.-L. Gautier-Lerestif, J.-L. Avril, and N. Lafforgue
Changes in staphylococcal cassette chromosome type and antibiotic resistance profile in methicillin-resistant Staphylococcus aureus isolates from a French hospital over an 11 year period
J. Antimicrob. Chemother., May 1, 2004; 53(5): 808 - 813.
[Abstract] [Full Text] [PDF]

Home page
 Antimicrob. Agents Chemother.Home page
D. Jabes, G. Candiani, G. Romano, C. Brunati, S. Riva, and M. Cavaleri
Efficacy of Dalbavancin against Methicillin-Resistant Staphylococcus aureus in the Rat Granuloma Pouch Infection Model
Antimicrob. Agents Chemother., April 1, 2004; 48(4): 1118 - 1123.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Microbiol.Home page
D. Harmsen, H. Claus, W. Witte, J. Rothganger, H. Claus, D. Turnwald, and U. Vogel
Typing of Methicillin-Resistant Staphylococcus aureus in a University Hospital Setting by Using Novel Software for spa Repeat Determination and Database Management
J. Clin. Microbiol., December 1, 2003; 41(12): 5442 - 5448.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.