Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (8)
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Qiang, Y. Z.
Right arrow Articles by Yi, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Qiang, Y. Z.
Right arrow Articles by Yi, G.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Journal of Antimicrobial Chemotherapy (2002) 49, 549-552
© 2002 The British Society for Antimicrobial Chemotherapy

Brief report

Use of a rapid mismatch PCR method to detect gyrA and parC mutations in ciprofloxacin-resistant clinical isolates of Escherichia coli

Yan Zhi Qianga, Tong Qina, Wang Fub, Wu Pei Chengb, Yang Sheng Lia and Gong Yia,*

a Shanghai Research Center of Biotechnology, SIBS, Chinese Academy of Sciences, 500 Cao Bao Road, Shanghai 200233; b Institute of Antibiotics, Medical Center of Fu Dan University, Shanghai, China

Four amino acid substitutions, two in GyrA and two in ParC subunits of DNA gyrase and topoisomerase IV, respectively, are commonly responsible for fluoroquinolone resistance in Escherichia coli. In this study, an economical and time-efficient mismatch amplification mutation assay (MAMA) PCR was developed to detect mutations in the chromosomal gyrA and parC genes causing these substitutions. One hundred and twenty-one clinical E. coli isolates were tested by this assay, and the results confirmed that accumulation of amino acid alterations in GyrA and ParC correlates closely with stepwise increases in the MIC of ciprofloxacin.

* Corresponding author. Tel: +86-21-6436-9607; Fax: +86-21-6470-0244; E-mail: yigong{at}srcb.ac.cn


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Appl. Environ. Microbiol.Home page
D. Kilmartin, D. Morris, C. O'Hare, G. Corbett-Feeney, and M. Cormican
Clonal Expansion May Account for High Levels of Quinolone Resistance in Salmonella enterica Serovar Enteritidis
Appl. Envir. Microbiol., May 1, 2005; 71(5): 2587 - 2591.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Microbiol.Home page
X. Yu, M. Susa, C. Knabbe, R. D. Schmid, and T. T. Bachmann
Development and Validation of a Diagnostic DNA Microarray To Detect Quinolone-Resistant Escherichia coli among Clinical Isolates
J. Clin. Microbiol., September 1, 2004; 42(9): 4083 - 4091.
[Abstract] [Full Text] [PDF]

Home page
 J Antimicrob ChemotherHome page
S. Eick, A. Schmitt, S. Sachse, K.-H. Schmidt, and W. Pfister
In vitro antibacterial activity of fluoroquinolones against Porphyromonas gingivalis strains
J. Antimicrob. Chemother., August 1, 2004; 54(2): 553 - 556.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.