Journal of Antimicrobial Chemotherapy (2002) 49, 261-267
© 2002 The British Society for Antimicrobial Chemotherapy
Relationship between blaSHV-12 and blaSHV-2a in Korea
a Department of Microbiology, College of Medicine, Dankook University, Cheonan; b Department of Microbiology, Kyungpook National University School of Medicine, 101, DongIn-2Ga, Taegu 700-422, South Korea
In contrast to the USA and Europe, where SHV-2, SHV-4 and SHV-5 are the prevalent extended-spectrum SHV enzymes, in Korea SHV-2a and SHV-12 are the most frequently identified extended-spectrum SHV enzymes. A 6.6 kb BamHI fragment containing the blaSHV-12 gene of strain K7746 isolated from one university hospital in Korea was cloned into the pCRScriptCAM vector. Sequencing of the constructed recombinant plasmid pK7746-C1 revealed that the immediate upstream sequence of the blaSHV-12 gene showed little similarity to the part of the prototype blaSHV-1 gene due to the insertion of an IS26 element next to the –10 region. Instead, the upstream sequences of blaSHV-12 retained 100% DNA identity with the part of plasmid pMPA2a from Klebsiella pneumoniae KPZU-3 carrying blaSHV-2a. The restriction map of the inserted 6.6 kb DNA fragment of plasmid pK7746-C1 was also homologous to that of plasmid pMPA2a, suggesting a common lineage of blaSHV-12 and blaSHV-2a. We also studied, using PCR, the upstream non-coding region of several SHV ß-lactamase genes for the presence of IS26 sequence. The flanking IS26 sequence in the immediate upstream region of the blaSHV gene was not detected in five standard strains producing SHV-1, SHV-2, SHV-3, SHV-4 or SHV-5. However, IS26 was detected in all 69 clinical strains producing SHV-2a or SHV-12 isolated from three university hospitals in Korea during 1993–1999. The above findings suggest a direct evolution of SHV-12 from SHV-2a, not from SHV-2 to -5, and it is considered to be one of the reasons for the absolute predominance of SHV-2a and SHV-12 in Korea.
* Corresponding author. Tel: +82-53-420-6951; Fax: +82-53-427-5664; E-mail: dtcho{at}knu.ac.kr
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