Journal of Antimicrobial Chemotherapy (2001) 48, 631-640
© 2001 The British Society for Antimicrobial Chemotherapy
Tetracycline resistance genes in isolates of Pasteurella multocida, Mannheimia haemolytica, Mannheimia glucosida and Mannheimia varigena from bovine and swine respiratory disease: intergeneric spread of the tet(H) plasmid pMHT1
a Institut für Tierzucht und Tierverhalten der Bundesforschungsanstalt für Landwirtschaft (FAL), Dörnbergstrasse 25–27, 29223 Celle, Germany; b Worldwide Animal Health Product Development, Pharmacia Animal Health, Kalamazoo, MI 49001; c Infectious Diseases Biology, Pharmacia Corporation, Kalamazoo, MI 49001, USA
Tetracycline-resistant isolates of Pasteurella multocida and Mannheimia spp. from respiratory diseases in cattle and swine were investigated for the classes of tet gene and their chromosomal or plasmid location. The 34 isolates comprised eight P. multocida, 23 Mannheimia haemolytica, two Mannheimia varigena and a single Mannheimia glucosida isolate. Identification of the tet genes was achieved by PCR analysis and hybridization with specific probes. Transformation and hybridization experiments served to confirm the plasmid location of tet genes. Selected tet genes and their adjacent regions were sequenced. The tet genes tet(B), tet(G) and tet(H) were detected. The gene tet(H) was present in 26 isolates. The 4.4 kb tet(H)-carrying plasmid pMHT1 was detected in six isolates representing all four species. In the remaining 28 isolates, copies of tet(B), tet(G) and tet(H) were identified as chromosomal. No correlation between the tet gene type and the MIC of tetracycline, or between the number of tet gene copies and the MIC of tetracycline was observed. Tetracycline resistance in P. multocida and Mannheimia spp. is mediated by at least three different tet genes. A new type of tet(H)- carrying plasmid, pMHT1, was identified. The detection of pMHT1 in M. glucosida and M. varigena is the first report of resistance plasmids in isolates of these two species. For the first time, tet(G) genes were detected in members of the family Pasteurellaceae.
* Corresponding author. Tel: +49-5141-384673, Fax: +49-5141-381849; E-mail: stefan.schwarz{at}fal.de
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  A. San Millan, J. A. Escudero, B. Gutierrez, L. Hidalgo, N. Garcia, M. Llagostera, L. Dominguez, and B. Gonzalez-Zorn Multiresistance in Pasteurella multocida Is Mediated by Coexistence of Small Plasmids Antimicrob. Agents Chemother., August 1, 2009; 53(8): 3399 - 3404. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Kehrenberg, J. Wallmann, and S. Schwarz Molecular analysis of florfenicol-resistant Pasteurella multocida isolates in Germany J. Antimicrob. Chemother., November 1, 2008; 62(5): 951 - 955. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Blanco, K. Kadlec, C. B. Gutierrez Martin, A. J. M. de la Fuente, S. Schwarz, and J. Navas Nucleotide sequence and transfer properties of two novel types of Actinobacillus pleuropneumoniae plasmids carrying the tetracycline resistance gene tet(H) J. Antimicrob. Chemother., October 1, 2007; 60(4): 864 - 867. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Kehrenberg and S. Schwarz Distribution of Florfenicol Resistance Genes fexA and cfr among Chloramphenicol-Resistant Staphylococcus Isolates Antimicrob. Agents Chemother., April 1, 2006; 50(4): 1156 - 1163. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Blanco, C. B. Gutierrez-Martin, E. F. Rodriguez-Ferri, M. C. Roberts, and J. Navas Distribution of Tetracycline Resistance Genes in Actinobacillus pleuropneumoniae Isolates from Spain Antimicrob. Agents Chemother., February 1, 2006; 50(2): 702 - 708. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Kadlec, C. Kehrenberg, and S. Schwarz Molecular basis of resistance to trimethoprim, chloramphenicol and sulphonamides in Bordetella bronchiseptica J. Antimicrob. Chemother., September 1, 2005; 56(3): 485 - 490. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Kehrenberg, B. Catry, F. Haesebrouck, A. de Kruif, and S. Schwarz tet(L)-mediated tetracycline resistance in bovine Mannheimia and Pasteurella isolates J. Antimicrob. Chemother., August 1, 2005; 56(2): 403 - 406. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Kehrenberg, B. Catry, F. Haesebrouck, A. de Kruif, and S. Schwarz Novel Spectinomycin/Streptomycin Resistance Gene, aadA14, from Pasteurella multocida Antimicrob. Agents Chemother., July 1, 2005; 49(7): 3046 - 3049. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Blickwede and S. Schwarz Molecular analysis of florfenicol-resistant Escherichia coli isolates from pigs J. Antimicrob. Chemother., January 1, 2004; 53(1): 58 - 64. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Kehrenberg, N. T. T. Tham, and S. Schwarz New Plasmid-Borne Antibiotic Resistance Gene Cluster in Pasteurella multocida Antimicrob. Agents Chemother., September 1, 2003; 47(9): 2978 - 2980. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. D. Miranda, C. Kehrenberg, C. Ulep, S. Schwarz, and M. C. Roberts Diversity of Tetracycline Resistance Genes in Bacteria from Chilean Salmon Farms Antimicrob. Agents Chemother., March 1, 2003; 47(3): 883 - 888. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Bosch, M. E. Garrido, M. Llagostera, A. M. Perez de Rozas, I. Badiola, and J. Barbe Characterization of the Pasteurella multocida hgbA Gene Encoding a Hemoglobin-Binding Protein Infect. Immun., November 1, 2002; 70(11): 5955 - 5964. [Abstract] [Full Text] [PDF]
|
|