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Journal of Antimicrobial Chemotherapy (2001) 48, 287-289
© 2001 The British Society for Antimicrobial Chemotherapy

Brief report

A comparison of culture and PCR to determine the prevalence of ampicillin-resistant bacteria in the faecal flora of general practice patients

John Heritagea,*, Nicola Ransomeb, Philip A. Chambersa and Mark H. Wilcoxa,b

a Division of Microbiology, University of Leeds, Leeds LS2 9JT; b Department of Microbiology, The General Infirmary at Leeds, Leeds, UK

Of 95 faecal specimens containing bacterial DNA amplified by PCR, 24% contained cultivable bacteria that were resistant to high-level ampicillin. When these samples were examined by PCR using primers to amplify the blaTEM gene, the number of positive samples identified increased significantly to 49 (52%). These results indicate that ampicillin resistance is common in the study population. Furthermore, the blaTEM gene is more common than indicated by the recovery of resistant bacteria in culture. This points to potential anomalies in the assessment of the prevalence of resistance when relying on recovery of resistant bacteria by culture.

* Corresponding author. Tel: +44-113-233-5592; Fax: +44-113-233-5638; E-mail: j.heritage{at}leeds.ac.uk


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