Journal of Antimicrobial Chemotherapy (2001) 48, 7-13
© 2001 The British Society for Antimicrobial Chemotherapy
Antimicrobial effects of positively charged surfaces on adhering Gram-positive and Gram-negative bacteria
a Department of Biomedical Engineering, University of Groningen, Antonius Deusinglaan 1, 9713 AV Groningen; b Department of Chemical Technology and Institute of Biomedical Technology, University of Twente, PO Box 217, 7500 AE Enschede, The Netherlands
The infection of biomaterials is determined by an interplay of adhesion and surface growth of the infecting organisms. In this study, the antimicrobial effects on adhering bacteria of a positively charged poly(methacrylate) surface (
potential +12 mV) were compared with those of negatively charged poly(methyl methacrylate) (–12 mV) and a highly negatively charged poly(methacrylate) (–18 mV) surface. Initial adhesion of Staphylococcus aureus ATCC 12600, Staphylococcus epidermidis HBH2 102, Escherichia coli O2K2 and Pseudomonas aeruginosa AK1 to these surfaces was measured in a parallel plate flow chamber in phosphate-buffered saline. Adhering bacteria were allowed to multiply by perfusing the flow chamber with growth medium. All bacteria adhered most rapidly to the positively charged surface, but there was no subsequent surface growth of the Gram-negative strains. On the negatively charged surfaces, despite a slower initial adhesion, surface growth of the adhering bacteria was exponential for both Gram-positive and Gram-negative strains. These results suggest that positively charged biomaterial surfaces exert an antimicrobial effect on adhering Gram-negative bacteria, but not on Gram-positive ones.
* Corresponding author. Tel: +31-50-3633140; Fax: +31-50-3633159; E-mail: h.j.busscher{at}med.rug.nl
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