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Journal of Antimicrobial Chemotherapy (2001) 47, 463-465
© 2001 The British Society for Antimicrobial Chemotherapy

Brief report

SHV-27, a novel cefotaxime-hydrolysing ß-lactamase, identified in Klebsiella pneumoniae isolates from a Brazilian hospital

John E. Corkilla,*, Luis E. Cuevasb, Ricardo Q. Gurgelc, Julie Greensilla and C. Anthony Harta

a Royal Liverpool University Hospital, Department of Medical Microbiology, Liverpool L7 8XP; b Liverpool School of Tropical Medicine, University of Liverpool, Liverpool L3 5QA, UK; c Department of Paediatrics, Federal University of Sergipe, Aracaju, Brazil

From a collection of cefotaxime-resistant Klebsiella pneumoniae isolated from neonatal blood culture specimens in a maternity hospital in Aracaju, Brazil, two isolates (strains KPBRZ-842 and -843, indistinguishable by pulsed-field gel electrophoresis) were found to produce ß-lactamases with isoelectric points (pI) of 5.4 and 8.2, respectively. Using a gel overlay method, cefotaxime hydrolysis was shown to be associated with the pI 8.2 protein. Nucleotide sequencing of the gene encoding the pI 8.2 ß-lactamase revealed a blaSHV-ESBL-type gene differing from the gene encoding SHV-1 by three silent point mutations, and a fourth that resulted in an amino acid substitution, aspartate for glycine, at position 156. This novel SHV-type extended-spectrum ß-lactamase is designated SHV-27.

* Corresponding author. Tel: +44-151-706-4410 ext. 4421; Fax: +44-151-706-5849; E-mail: jecmm{at}liverpool.ac.uk


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