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Journal of Antimicrobial Chemotherapy (2001) 47, 285-296
© 2001 The British Society for Antimicrobial Chemotherapy

Polymorphism of Neisseria meningitidis penA gene associated with reduced susceptibility to penicillin

Aude Antignaca, Paula Krizb, Georgina Tzanakakic, Jean-Michel Alonsoa and Muhamed-Kheir Tahaa,*

a Unité des Neisseria and Centre National de Référence des Méningocoques, Institut Pasteur, 28 Rue du Dr Roux, 75724 Paris cedex 15, France; b National Reference Laboratory for Meningococcal Infections, NIPH, Prague, Czech Republic; c National Meningococcal Reference Laboratory, 196 Alexandras Avenue, 11521 Athens, Greece

We studied polymorphism of penA (which encodes penicillin-binding protein 2) in 13 strains of Neisseria meningitidis susceptible to penicillin (penS) and 12 strains with reduced susceptibility to penicillin (penI). These strains differed in geographical origin. Serological and genetic typing showed that they were highly diverse and belonged to several genetic lineages. Restriction analysis and DNA sequencing of penA showed that all penS strains had the same penA allele regardless of genetic group, whereas penI strains harboured various penA alleles. Transformation with amplicons of penA and genomic DNA from several penI strains conferred the penI phenotype on a penS strain. Thus, reduction in susceptibility to penicillin is directly related to changes in penA and analysis of penA polymorphisms could be used as a reliable tool for characterizing meningococcal strains in terms of their susceptibility to penicillin.

* Corresponding author. Tel: +33-1-45688438; Fax: +33-1-45688338; E-mail: mktaha{at}pasteur.fr


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