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Journal of Antimicrobial Chemotherapy (2001) 47, 187-189
© 2001 The British Society for Antimicrobial Chemotherapy

Brief report

Evaluation of the Mastalex latex agglutination test for methicillin resistance in Staphylococcus aureus grown on different screening media

Derek F. J. Brown,* and Enid Walpole

Clinical Microbiology and Public Health Laboratory, Addenbrooke's Hospital, Hills Road, Cambridge CB2 2QW, UK

The Mastalex MRSA latex agglutination method was evaluated with 52 methicillin-resistant and 27 methicillin-susceptible strains of Staphylococcus aureus grown on various media. All tests were correct with colonies grown on blood agar with or without oxacillin 2 mg/L. Tests on colonies grown on mannitol salt agar were less reliable, although addition of oxacillin 2 mg/L improved performance. One of the 26 MRSA which grew on Baird–Parker medium with 8 mg/L ciprofloxacin gave a false-negative result. Agglutination was faster when strains were grown on media with oxacillin. The method would be particularly useful for urgent confirmation of resistance.

* Corresponding author. Tel: +44-1223-257020; Fax: +44-1223-242775; E-mail: dfjb2{at}cam.ac.uk


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