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Journal of Antimicrobial Chemotherapy (2000) 46, 997-1000
© 2000 The British Society for Antimicrobial Chemotherapy

Comparison of the Etest and microdilution method for antifungal susceptibility testing of Cryptococcus neoformans to four antifungal agents

A. I. Aller, E. Martín-Mazuelos*, M. J. Gutiérrez, S. Bernal, M. Chávez and F. J. Recio

Servicio de Microbiología, Hospital Universitario de Valme, Carretera de Cádiz s/n, E-41014 Seville, Spain

We performed a prospective study to compare the Etest and the microdilution method (NCCLS guidelines) for determining the MICs of fluconazole, itraconazole, flucytosine and amphotericin B for 35 strains of Cryptococcus neoformans. For the microdilution method (MDM) RPMI 1640 medium with 2% glucose was used for fluconazole, itraconazole and flucytosine, and Antibiotic Medium 3 for amphotericin B. For the Etest, RPMI 1640 medium with 2% glucose and solidified with 1.5% agar was used for the four antifungal agents. Amphotericin B was also tested on Antibiotic Medium 3 solidified with 1.5% agar. Fluconazole and flucytosine MICs by the Etest showed good correlation with the broth MDM (81.1 and 89.2% agreement within two dilutions, respectively). With the tested population of itraconazole- and amphotericin B-susceptible isolates, the MIC agreement for itraconazole was 54%; amphotericin B showed the lowest agreement (8.1% on Antibiotic Medium 3 and 13.5% on RPMI).

* Corresponding author. Tel: +34-95-4596352; Fax: +34-95-4596395; E-mail: micemm{at}valme.sas.cica.es


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