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Journal of Antimicrobial Chemotherapy (2000) 46, 879-884
© 2000 The British Society for Antimicrobial Chemotherapy

A TEM-2 ß-lactamase encoded on an active Tn1-like transposon in the genome of a clinical isolate of Stenotrophomonas maltophilia

Matthew B. Avison*, Charlotte J. von Heldreich, Catherine S. Higgins, Peter M. Bennett and Timothy R. Walsh

Bristol Centre for Antimicrobial Research and Evaluation (BCARE), Department of Pathology and Microbiology, School of Medical Sciences, University of Bristol, Bristol BS8 1TD, UK

A constitutively expressed ß-lactamase gene from a clinical isolate of Stenotrophomonas maltophilia, J675Ia, has been cloned. Its DNA sequence is almost identical to that of blaTEM2 (one nucleotide change) and the expressed enzyme is a Bush type 2a penicillinase with an amino acid sequence identical to that of TEM-2. The blaTEM gene was present within a novel Tn1/Tn3-type transposon in the genome of isolate J675Ia and the transposon was able to mobilize blaTEM on to the broad host-range conjugative plasmid, R388. When transferred to an Escherichia coli recipient, R388::Tn conferred high-level ampicillin resistance. This represents the first identification of a TEM ß-lactamase in S. maltophilia and the first evidence that this important clinical pathogen is able to act as a reservoir for mobile ß-lactamase genes in the hospital environment.

* Corresponding author. Tel: +44-117-9287541; Fax: +44-117-9287896; Email: Matthewb.Avison{at}bris.ac.uk


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