Journal of Antimicrobial Chemotherapy (2000) 46, 617-620
© 2000 The British Society for Antimicrobial Chemotherapy
Brief report |
In vivo deletion of the methicillin resistance mec region from the chromosome of Staphylococcus aureus strains
a Department of Microbiology, Erasme Hospital, Université Libre de Bruxelles, route de Lennik 808, B-1070 Brussels, Belgium; b Department of Microbiology, Medical School, University of Athens, Athens, Greece; c Department of Microbiology, Cliniques Universitaires UCL de Mont-Godinne, Yvoir, Belgium; d Applied Genetics, ULB, Nivelles, Belgium
Two sets of Staphylococcus aureus isolates recovered from two patients exhibited similar susceptibility profiles except for oxacillin susceptibility (MSSA) or resistance (MRSA). SmaI macrorestriction and inter-IS256 PCR analysis showed patterns closely related to the Belgian epidemic MRSA clone 1 in each pair of MSSA/MRSA strains. Loss of one large SmaI DNA fragment and concurrent gain of a smaller fragment in the MSSA isolates was observed. The mecA sequence present in the MRSA was absent in the MSSA variant. Therefore, in vivo deletion of the mec region may occur in some lineages of S. aureus more frequently than previously thought.
* Corresponding author. Tel: +32-2-5554517; Fax: +32-2-5556459; E-mail: ariane.deplano{at}ulb.ac.be
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