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Journal of Antimicrobial Chemotherapy (2000) 46, 363-368
© 2000 The British Society for Antimicrobial Chemotherapy

Inactivation of a hepadnavirus by electrolysed acid water

Masami Tagawaa,*, Taketo Yamaguchia, Osamu Yokosukaa, Shoichi Matsutania, Toyoyuki Maedab and Hiromitsu Saishoa

a First Department of Medicine, School of Medicine, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba 260-8670, Japan and b Department of Medical Instruments, Kaigen Co. Ltd, Osaka, Japan

Glutaraldehyde is used as a disinfectant for endoscopes, but is an irritant and so should be replaced by an alternative. Electrolysed acid water (EAW) has a bactericidal effect, and an endoscopic washing device using EAW has been developed in Japan. To investigate the effect of EAW on the infectivity of viruses, we treated duck hepatitis B virus (DHBV), which has similar properties to hepatitis B virus, with EAW, and determined the number of remaining infectious virus particles in a bioassay system. One-day-old Pekin ducks were inoculated with duck serum containing 105.5 ID50 DHBV; the serum had previously been incubated with 100 volumes of EAW or ion-exchanged water at room temperature for 7 min. DHBV infection was indicated by detection of viral DNA in duck serum samples 1–8 weeks after inoculation. Treatment of serum with EAW diminished DHBV infectivity whereas treatment with ion-exchanged water did not. The virus load was estimated to have been reduced to 101–103 ID50 during the first 1 min and to <100.5 ID50 in the next 6 min of incubation when compared with the control. Thus, EAW directly inactivates DHBV and its clinical application is recommended.

* Corresponding author. Tel: +81-43-226-2086; Fax: +81-43-226-2088; E-mail: masami{at}med.m.chiba-u.ac.jp


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