Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (11)
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Yanagihara, K.
Right arrow Articles by Kohno, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yanagihara, K.
Right arrow Articles by Kohno, S.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Journal of Antimicrobial Chemotherapy (2000) 46, 69-72
© 2000 The British Society for Antimicrobial Chemotherapy

Combination therapy for chronic Pseudomonas aeruginosa respiratory infection associated with biofilm formation

Katsunori Yanagihara*, Kazunori Tomono, Toyomitsu Sawai, Misuzu Kuroki, Yukihiro Kaneko, Hideaki Ohno, Yasuhito Higashiyama, Yoshitsugu Miyazaki, Yoichi Hirakata, Shigefumi Maesaki, Jun-ichi Kadota, Takayoshi Tashiro and Shigeru Kohno

The Second Department of Internal Medicine, Nagasaki University School of Medicine, 1-7-1 Sakamoto, Nagasaki, 852-8501 Japan

There had been no reports of investigations into biofilms in chronic respiratory infection in vivo. Recently, we established a new murine model of chronic respiratory infection with Pseudomonas aeruginosa. In the present study, we examined the bacteriological effect of combined clarithromycin and levofloxacin against chronic respiratory infection with P. aeruginosa. Scanning electron micrograph of the surface of the catheter intubated in mouse bronchus for 7 days demonstrated in vivo formation of a biofilm containing blood cells, complex fibrous structures and bacteria. Treatment with either clarithromycin alone or levofloxacin alone had no statistical effect on the number of viable bacteria in lung. The combined use of both drugs resulted in a significant decrease in the number of viable bacteria. The present experiment demonstrates that the newly established murine model was useful to investigate the treatment of biofilm-associated chronic respiratory infection with P. aeruginosa, and combination therapy with clarithromycin and levofloxacin was effective in biofilm-associated chronic respiratory infection.

* Corresponding author. Tel: +81-95-849-7273; Fax: +81-95-849-7285.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J Antimicrob ChemotherHome page
Y. Morinaga, K. Yanagihara, S. Nakamura, K. Yamamoto, K. Izumikawa, M. Seki, H. Kakeya, Y. Yamamoto, Y. Yamada, S. Kohno, et al.
In vivo efficacy and pharmacokinetics of tomopenem (CS-023), a novel carbapenem, against Pseudomonas aeruginosa in a murine chronic respiratory tract infection model
J. Antimicrob. Chemother., December 1, 2008; 62(6): 1326 - 1331.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
L. M. Mashburn, A. M. Jett, D. R. Akins, and M. Whiteley
Staphylococcus aureus Serves as an Iron Source for Pseudomonas aeruginosa during In Vivo Coculture
J. Bacteriol., January 15, 2005; 187(2): 554 - 566.
[Abstract] [Full Text] [PDF]

Home page
 J Antimicrob ChemotherHome page
G. W. Amsden
Anti-inflammatory effects of macrolides--an underappreciated benefit in the treatment of community-acquired respiratory tract infections and chronic inflammatory pulmonary conditions?
J. Antimicrob. Chemother., January 1, 2005; 55(1): 10 - 21.
[Abstract] [Full Text] [PDF]

Home page
 Antimicrob. Agents Chemother.Home page
T. Nagata, H. Mukae, J. Kadota, T. Hayashi, T. Fujii, M. Kuroki, R. Shirai, K. Yanagihara, K. Tomono, T. Koji, et al.
Effect of Erythromycin on Chronic Respiratory Infection Caused by Pseudomonas aeruginosa with Biofilm Formation in an Experimental Murine Model
Antimicrob. Agents Chemother., June 1, 2004; 48(6): 2251 - 2259.
[Abstract] [Full Text] [PDF]

Home page
 Antimicrob. Agents Chemother.Home page
E. J. Giamarellos-Bourboulis, T. Adamis, G. Laoutaris, L. Sabracos, V. Koussoulas, M. Mouktaroudi, D. Perrea, P. E. Karayannacos, and H. Giamarellou
Immunomodulatory Clarithromycin Treatment of Experimental Sepsis and Acute Pyelonephritis Caused by Multidrug-Resistant Pseudomonas aeruginosa
Antimicrob. Agents Chemother., January 1, 2004; 48(1): 93 - 99.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
Y. Kaneko, K. Yanagihara, M. Seki, M. Kuroki, Y. Miyazaki, Y. Hirakata, H. Mukae, K. Tomono, J.-i. Kadota, and S. Kohno
Clarithromycin inhibits overproduction of muc5ac core protein in murine model of diffuse panbronchiolitis
Am J Physiol Lung Cell Mol Physiol, October 1, 2003; 285(4): L847 - L853.
[Abstract] [Full Text] [PDF]

Home page
 J Med MicrobiolHome page
K. Yanagihara, K. Tomono, Y. Kaneko, Y. Miyazaki, K. Tsukamoto, Y. Hirakata, H. Mukae, J.-i. Kadota, I. Murata, and S. Kohno
Role of elastase in a mouse model of chronic respiratory Pseudomonas aeruginosa infection that mimics diffuse panbronchiolitis
J. Med. Microbiol., June 1, 2003; 52(6): 531 - 535.
[Abstract] [Full Text] [PDF]

Home page
 J Antimicrob ChemotherHome page
K. Yanagihara, K. Tomono, Y. Imamura, Y. Kaneko, M. Kuroki, T. Sawai, Y. Miyazaki, Y. Hirakata, H. Mukae, J.-I. Kadota, et al.
Effect of clarithromycin on chronic respiratory infection caused by Pseudomonas aeruginosa with biofilm formation in an experimental murine model
J. Antimicrob. Chemother., May 1, 2002; 49(5): 867 - 870.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.