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Journal of Antimicrobial Chemotherapy (2000) 45, 677-680
© 2000 The British Society for Antimicrobial Chemotherapy


Brief reports

Characterization of vancomycin-resistant and vancomycin-susceptible Enterococcus faecium isolates from humans, chickens and pigs by RiboPrinting and pulsed-field gel electrophoresis

Anette Marie Hammeruma,*, Vivian Fussingb, Frank Møller Aarestrupa and Henrik Caspar Wegenera

a Danish Veterinary Laboratory, Bülowsvej 27, DK-1790 Copenhagen V; b Statens Serum Institut, Artillerivej 5, DK-2300 Copenhagen S, Denmark

Forty-eight vancomycin-resistant and 35 vancomycin-sensitive Danish Enterococcus faecium isolates obtained from pigs, chickens and humans, as well as the human vanA reference isolate BM4147, were characterized by EcoRI RiboPrinting and SmaI pulsed-field gel electrophoresis. RiboPrinting of the 84 isolates yielded 40 types whereas PFGE-typing yielded 57 types discriminated by differences in more than three bands. By molecular typing, both clonal spread of E. faecium as well as horizontal transmission of Tn1546 between animals and humans was supported. Furthermore, it was found that the population of E. faecium spreads freely between the animal and human reservoir.

* Corresponding author. Tel: +45-35-30-01-00; Fax: +45-35-30-01-20; E-mail: aha{at}svs.dk


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