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Journal of Antimicrobial Chemotherapy (2000) 45, 521-524
© 2000 The British Society for Antimicrobial Chemotherapy

Brief reports

In vitro selected fluoroquinolone-resistant mutants of Citrobacter freundii: analysis of the quinolone resistance acquisition

María del Mar Tavíoa,*, Jordi Vilab, Joaquím Ruizb, Gianfranco Amicosantec, Nicola Franceschinic, Antonio M. Martín-Sáncheza and María Teresa Jiménez de Antab

a Microbiology, Department of Clinical Sciences, School of Medicine, University of Las Palmas de Gran Canaria, Dr Pasteur, 35080 Las Palmas de Gran Canaria; b Department of Microbiology, Hospital Clinic, School of Medicine, University of Barcelona, Villarroel, 170, 08036 Barcelona, Spain; c Department of Science and Biomedical Technology, Universitá degli Studi dell’Aquila, Via Vetoio, Loc. Coppito, 67100 L’Aquila, Italy

Ten quinolone-resistant mutants of Citrobacter freundii, which were selected in vitro with fluoroquinolones from two clinical isolates, were studied. The parent isolates were susceptible to quinolones in spite of showing a single substitution in the GyrB (His-417 -> Leu). No change was observed in the outer membrane proteins or in the lipopolysaccharide in any of the ten mutants studied with respect to their parent isolates. The development of quinolone resistance in selected mutants was associated with the appearance of a substitution in the GyrA (Thr-83 -> Ile) in nine of the ten mutants plus enhanced active efflux in all of them.

* Corresponding author. Tel: +34-928-453405/451459; Fax: +34-928-451416; E-mail: mtavio{at}infovia.ulpgc.es


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