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Journal of Antimicrobial Chemotherapy (2000) 45, 517-519
© 2000 The British Society for Antimicrobial Chemotherapy

Brief reports

Combinatorial biochemistry and shuffling of TEM, SHV and Streptomyces albus omega loops in PSE-4 class A ß-lactamase

François Sanschagrina, Esther Thériaulta, Yves Sabbagha, Normand Voyerb and Roger C. Levesquea,*

a Microbiologie Moléculaire & Génie des Protéines, Sciences de la Vie et de la Santé, Faculté de Médecine, Pavillon Charles-Eugène Marchand, Université Laval; b Département de Chimie, Pavillon Alexandre-Vachon, Université Laval, Ste-Foy, Québec, Canada G1K 7P4

The class A PSE-4 ß-lactamase was used for studying the importance of amino acids in the omega ({Omega}) loop and its interactions for hydrolysis of ß-lactam antibiotics. By cassette mutagenesis, we replaced the amino acids 163–179 {Omega} loop in PSE-4 with TEM-1, SHV-1 and Streptomyces albus G ß-lactamase {Omega} loops. Phenotypic analysis of Escherichia coli recombinants expressing the {Omega} loop PSE-4 mutant enzymes gave MICs and kinetic data similar to those of wild-type PSE-4.

* Corresponding author. Tel: +1-418-656-3070; Fax: +1-418-656-7176; E-mail: rclevesq{at}rsvs.ulaval.ca.


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