Journal of Antimicrobial Chemotherapy (2000) 45, 349-351
© 2000 The British Society for Antimicrobial Chemotherapy
Brief reports |
First identification of an SHV-12 extended-spectrum ß-lactamase in Klebsiella pneumoniae isolated in Italy
a Dipartimento di Scienze Microbiologiche, Università di Catania, via Androne 81, 95124 Catania; b Dipartimento di Scienze e Tecnologie Biomediche, Università dell'Aquila, via Vetoio, L'aquila, Italy
A clinical isolate of Klebsiella pneumoniae highly resistant to third- and fourth-generation cephalosporins, cephamycins and aminoglycosides, was isolated in 1991 from urine. Analysis of a crude extract showed the presence of three ß-lactamases with isoelectric points of 6.6, 7.5 and 8.2. The enzyme with pI 8.2 was transferred by conjugation into Escherichia coli K-12 J53 and was responsible for the resistance to third-generation cephalosporins and monobactams, but not to other antibiotics. Kinetic studies of partially purified ß-lactamase from the transconjugant strain confirmed that the enzyme was able to hydrolyse ceftazidime, cefotaxime and aztreonam but not cephamycins. Analysis of the transconjugant showed the presence of two small non-conjugative plasmids of 14 and 6 kb. A polymerase chain reaction was performed using primers specific for the blaSHV gene and a fragment of the expected size (about 961 bp) was obtained with both the K. pneumoniae clinical isolate and the transconjugant. Nucleotide sequence analysis of the fragment showed that it encoded the enzyme SHV-12, derived from SHV-5 (with Gln-35 to Leu). This is the first report of an SHV-12-like enzyme isolated in Italy.
* Corresponding author. Tel: +39-95-311352; Fax: +39-95-325032; E-mail: stefanis{at}mbox.unict.it
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  L. Villa, C. Mammina, V. Miriagou, L. S. Tzouvelekis, P. T. Tassios, A. Nastasi, and A. Carattoli Multidrug and Broad-Spectrum Cephalosporin Resistance among Salmonella enterica Serotype Enteritidis Clinical Isolates in Southern Italy J. Clin. Microbiol., July 1, 2002; 40(7): 2662 - 2665. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Perilli, E. Dell'Amico, B. Segatore, M. R. de Massis, C. Bianchi, F. Luzzaro, G. M. Rossolini, A. Toniolo, G. Nicoletti, and G. Amicosante Molecular Characterization of Extended-Spectrum {beta}-Lactamases Produced by Nosocomial Isolates of Enterobacteriaceae from an Italian Nationwide Survey J. Clin. Microbiol., February 1, 2002; 40(2): 611 - 614. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Teshager, L. Domínguez, M. A. Moreno, Y. Saénz, C. Torres, and S. Cardeñosa Isolation of an SHV-12 beta -Lactamase-Producing Escherichia coli Strain from a Dog with Recurrent Urinary Tract Infections Antimicrob. Agents Chemother., December 1, 2000; 44(12): 3483 - 3484. [Full Text]
|
|