Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (22)
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Tattawasart, U.
Right arrow Articles by Russell, A. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Tattawasart, U.
Right arrow Articles by Russell, A. D.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Journal of Antimicrobial Chemotherapy (2000) 45, 145-152
© 2000 The British Society for Antimicrobial Chemotherapy

Cytological changes in chlorhexidine-resistant isolates of Pseudomonas stutzeri

Unchalee Tattawasarta, A. C. Hannb, J.-Y. Maillarda, J. R. Furra and A. D. Russella,*

a Welsh School of Pharmacy, and b School of Molecular and Medical Biosciences, Cardiff University, Cardiff CF10 3XF, UK

Transmission electron microscopy (TEM), scanning electron microscopy (SEM) and energy-dispersive analysis of X-ray (EDAX) have been used to examine chlorhexidine diacetate (CHA)-sensitive and -resistant isolates of Pseudomonas stutzeri and to determine the effects of CHA on the cells. Significant differences were observed in the structure, size and elemental composition of CHA-sensitive and -resistant cells. Treatment with CHA produced considerably greater changes in CHA-sensitive cells, with widespread peeling of the outer membrane, a substantial loss of cytoplasmic electron-dense material and extensive lysis. Cells from the resistant isolates showed no blebbing of the outer membrane and no structural damage. X-ray mapping confirmed the difference in CHA uptake between CHA-sensitive and CHA-resistant cells. It is proposed that changes in the outer membrane form a major mechanism of resistance to CHA in P. stutzeri.

* Corresponding author. Tel: +44-1222-875812; Fax: +44-1222-874149; E-mail: russellD2{at}cardiff.ac.uk


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J Antimicrob ChemotherHome page
K. M. Ho and E. Litton
Use of chlorhexidine-impregnated dressing to prevent vascular and epidural catheter colonization and infection: a meta-analysis
J. Antimicrob. Chemother., August 1, 2006; 58(2): 281 - 287.
[Abstract] [Full Text] [PDF]

Home page
 Microbiol. Mol. Biol. Rev.Home page
J. Lalucat, A. Bennasar, R. Bosch, E. Garcia-Valdes, and N. J. Palleroni
Biology of Pseudomonas stutzeri
Microbiol. Mol. Biol. Rev., June 1, 2006; 70(2): 510 - 547.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Microbiol. Rev.Home page
G. Kampf and A. Kramer
Epidemiologic Background of Hand Hygiene and Evaluation of the Most Important Agents for Scrubs and Rubs
Clin. Microbiol. Rev., October 1, 2004; 17(4): 863 - 893.
[Abstract] [Full Text] [PDF]

Home page
 J Antimicrob ChemotherHome page
C. E. Codling, B. V. Jones, E. Mahenthiralingam, A. D. Russell, and J.-Y. Maillard
Identification of genes involved in the susceptibility of Serratia marcescens to polyquaternium-1
J. Antimicrob. Chemother., August 1, 2004; 54(2): 370 - 375.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Microbiol. Rev.Home page
P. Gilbert and A. J. McBain
Potential Impact of Increased Use of Biocides in Consumer Products on Prevalence of Antibiotic Resistance
Clin. Microbiol. Rev., April 1, 2003; 16(2): 189 - 208.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.