Journal of Antimicrobial Chemotherapy (2000) 45, 9-13
© 2000 The British Society for Antimicrobial Chemotherapy
Chromosomal ß-lactamase is packaged into membrane vesicles and secreted from Pseudomonas aeruginosa
a Institute for Medical Microbiology and Immunology, Panum Inst. 24.1, University of Copenhagen, Blegdamsvej 3, 2200 Copenhagen N, Denmark; b Department of Microbiology, University of Guelph, Guelph, Canada
Membrane vesicles were isolated from one ß-lactam-sensitive and three ß-lactam-resistant Pseudomonas aeruginosa clinical isolates from patients with cystic fibrosis. The presence of the chromosomally encoded ß-lactamase in the membrane vesicles was shown by electron microscopy and enzymatic studies. This is the first report of extracellular secretion of ß-lactamase in P. aeruginosa and it seems that the enzyme is packaged into membrane vesicles.
* Corresponding author. Tel: +45-3532-7899; Fax: +45-3532-7693; E-mail: O.Ciofu{at}immi.ku.dk
Present address. ROCHE Vitamins Inc., Research and Development, Building 102, B309, 340 Kingsley Street, Nutley, NJ 07110-1199, USA.
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