Journal of Antimicrobial Chemotherapy (2000) 45, 101-104
© 2000 The British Society for Antimicrobial Chemotherapy
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Detrimental effect of the combination of R164S with G238S in TEM-1 ß-lactamase on the extended-spectrum activity conferred by each single mutation
a Laboratory of Antimicrobial Agents, Department of Microbiology, Medical School, University of Athens, M. Asias 75, Athens 11527; b Department of Bacteriology, Hellenic Pasteur Institute, Athens, Greece
The non-naturally occurring TEM-1 ß-lactamase mutant R164S:G238S, as well as the R164S (TEM-12) and G238S (TEM-19) ß-lactamases, were constructed and expressed in Escherichia coli under isogenic conditions. Comparison of susceptibilities to ß-lactam antibiotics and substrate profiles showed that the combination of R164S with G238S drastically reduced the extended-spectrum activity of the respective single mutants.
* Corresponding author. Tel: +350-1-778-5638; Fax: +350-1-770-9180; E-mail: tzouvel{at}cc.uoa.gz
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