Journal of Antimicrobial Chemotherapy (1999) 44, 735-742
© 1999 The British Society for Antimicrobial Chemotherapy
Mechanisms involved in the development of resistance to fluoroquinolones in Escherichia coli isolates
a Microbiology, Department of Clinical Sciences, School of Medicine, University of Las Palmas de Gran Canaria, Dr. Pasteur s/n, 35080 Las Palmas; b Department of Microbiology, Hospital Clinic, School of Medicine, University of Barcelona, Villarroel 170, 08036 Barcelona, Spain
Eighteen quinolone-resistant isolates of Escherichia coli were selected by
exposing
ten
clinical isolates to increasing concentrations of norfloxacin and lomefloxacin. The mutant isolates
showed a multiple-antibiotic-resistance phenotype. All of them contained single mutations in gyrA consisting of the substitution of Ser-83
Leu (n = 14), Val (n
= 1) or Ala (n = 1) and the substitution of Asp-87Asn (n
= 2). Only one concomitant mutation in parC (Ser-80Arg) was detected.
Four
parent isolates exhibited a single mutation in gyrA which required 
12 mg/L of
norfloxacin
to be inhibited. Fluoroquinolone resistance, in the 18 quinolone-resistant mutants, was a result of
mutations affecting DNA gyrase plus decreased fluoroquinolone uptake. This latter mechanism of
resistance was a combined effect of an absence of OmpF and an increase in active efflux in eight
isolates, or an increased active efflux alone in the remaining ten selected mutants.
* Corresponding author. Tel:+34-28-451459; Fax: +34-28-451416; E-mail: mtavio{at}infovia.ulpgc.es
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  A. Cesaro, R. R. D. Bettoni, C. Lascols, A. Merens, C. J. Soussy, and E. Cambau Low selection of topoisomerase mutants from strains of Escherichia coli harbouring plasmid-borne qnr genes J. Antimicrob. Chemother., May 1, 2008; 61(5): 1007 - 1015. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Bore, M. Hebraud, I. Chafsey, C. Chambon, C. Skjaeret, B. Moen, T. Moretro, O. Langsrud, K. Rudi, and S. Langsrud Adapted tolerance to benzalkonium chloride in Escherichia coli K-12 studied by transcriptome and proteome analyses Microbiology, April 1, 2007; 153(4): 935 - 946. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Y. Han and R. A. Andrade Brevundimonas diminuta infections and its resistance to fluoroquinolones J. Antimicrob. Chemother., June 1, 2005; 55(6): 853 - 859. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. M Tavio, J. Vila, M. Perilli, L. T Casanas, L. Macia, G. Amicosante, and M. T Jimenez de Anta Enhanced active efflux, repression of porin synthesis and development of Mar phenotype by diazepam in two enterobacteria strains J. Med. Microbiol., November 1, 2004; 53(11): 1119 - 1122. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Wang, J. H. Tran, G. A. Jacoby, Y. Zhang, F. Wang, and D. C. Hooper Plasmid-Mediated Quinolone Resistance in Clinical Isolates of Escherichia coli from Shanghai, China Antimicrob. Agents Chemother., July 1, 2003; 47(7): 2242 - 2248. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Ruiz Mechanisms of resistance to quinolones: target alterations, decreased accumulation and DNA gyrase protection J. Antimicrob. Chemother., May 1, 2003; 51(5): 1109 - 1117. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Saenz, M. Zarazaga, L. Brinas, F. Ruiz-Larrea, and C. Torres Mutations in gyrA and parC genes in nalidixic acid-resistant Escherichia coli strains from food products, humans and animals J. Antimicrob. Chemother., April 1, 2003; 51(4): 1001 - 1005. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. P. Horcajada, J. Vila, A. Moreno-Martinez, J. Ruiz, J. A. Martinez, M. Sanchez, E. Soriano, and J. Mensa Molecular epidemiology and evolution of resistance to quinolones in Escherichia coli after prolonged administration of ciprofloxacin in patients with prostatitis J. Antimicrob. Chemother., January 1, 2002; 49(1): 55 - 59. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Ruiz, A. Jurado, E. Garcia-Mendez, F. Marco, L. Aguilar, M. T. Jimenez de Anta, and J. Vila Frequency of selection of fluoroquinolone-resistant mutants of Neisseria gonorrhoeae exposed to gemifloxacin and four other quinolones J. Antimicrob. Chemother., October 1, 2001; 48(4): 545 - 548. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Ciofu, V. Fussing, N. Bagge, C. Koch, and N. Hoiby Characterization of paired mucoid/non-mucoid Pseudomonas aeruginosa isolates from Danish cystic fibrosis patients: antibiotic resistance, {beta}-lactamase activity and RiboPrinting J. Antimicrob. Chemother., September 1, 2001; 48(3): 391 - 396. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Roychoudhury, T. L. Twinem, K. M. Makin, E. J. McIntosh, B. Ledoussal, and C. E. Catrenich Activity of non-fluorinated quinolones (NFQs) against quinolone-resistant Escherichia coli and Streptococcus pneumoniae J. Antimicrob. Chemother., July 1, 2001; 48(1): 29 - 36. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Oh, N. El Amin, T. Davies, P. C. Appelbaum, and C. Edlund gyrA Mutations Associated with Quinolone Resistance in Bacteroides fragilis Group Strains Antimicrob. Agents Chemother., July 1, 2001; 45(7): 1977 - 1981. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. M. Barnard and A. Maxwell Interaction between DNA Gyrase and Quinolones: Effects of Alanine Mutations at GyrA Subunit Residues Ser83 and Asp87 Antimicrob. Agents Chemother., July 1, 2001; 45(7): 1994 - 2000. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. S. Jellen-Ritter and W. V. Kern Enhanced Expression of the Multidrug Efflux Pumps AcrAB and AcrEF Associated with Insertion Element Transposition in Escherichia coli Mutants Selected with a Fluoroquinolone Antimicrob. Agents Chemother., May 1, 2001; 45(5): 1467 - 1472. [Abstract] [Full Text]
|
|
|
|
|
|
H. Wang, J. L. Dzink-Fox, M. Chen, and S. B. Levy Genetic Characterization of Highly Fluoroquinolone-Resistant Clinical Escherichia coli Strains from China: Role of acrR Mutations Antimicrob. Agents Chemother., May 1, 2001; 45(5): 1515 - 1521. [Abstract] [Full Text]
|
|
|
|
|
|
M. d. M. Tavio, J. Vila, J. Ruiz, G. Amicosante, N. Franceschini, A. M. Martin-Sanchez, and M. T. J. de Anta In vitro selected fluoroquinolone-resistant mutants of Citrobacter freundii: analysis of the quinolone resistance acquisition J. Antimicrob. Chemother., April 1, 2000; 45(4): 521 - 524. [Abstract] [Full Text] [PDF]
|
|