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Journal of Antimicrobial Chemotherapy (1999) 44, 557-560
© 1999 The British Society for Antimicrobial Chemotherapy

Brief report

In-vitro antifungal susceptibilities of Basidiobolus and Conidiobolus spp. strains

Josep Guarroa,*, Carme Aguilara and Isabel Pujolb

a Unitat de Microbiologia, Facultat de Medicina, Universitat Rovira i Virgili, Carrer Sant Llorenç, 21, 43201 Reus b Laboratori de Microbiologia, Hospital Universitari de Sant Joan de Reus, 43201 Reus, Tarragona, Spain

The in-vitro antifungal susceptibilities of nine isolates belonging to Basidiobolus spp. and seven to Conidiobolus spp. against six antifungals (amphotericin B, ketoconazole, miconazole, itraconazole, fluconazole and flucytosine) were tested. A broth microdilution method, generally following the NCCLS guidelines, was used. Inoculum concentrations of the order of 100 cfu/mL were obtained by culturing fungi in a broth medium (Czapeck broth supplemented with 2% Tween 80 and 0.07% agar). MICs and MFCs were highly variable and isolate-dependent, with the exception of those of flucytosine which were constantly very high. In general, however, Basidiobolus spp. displayed low MICs of fluconazole, itraconazole, ketoconazole and miconazole, and Conidiobolus spp. were resistant to all antifungals tested.

* Corresponding author. Tel: +34-977-759359; Fax: +34-977-759322; E-mail: umb{at}astor.urv.es


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