Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (13)
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Di Giorgio, C.
Right arrow Articles by Dumon, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Di Giorgio, C.
Right arrow Articles by Dumon, H.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Journal of Antimicrobial Chemotherapy (1999) 44, 71-76
© 1999 The British Society for Antimicrobial Chemotherapy

Flow cytometric assessment of amphotericin B susceptibility in Leishmania infantumisolates from patients with visceral leishmaniasis

C. Di Giorgioa,*, F. Faraut-Gambarellib, A. Imbertb, P. Minodierc, M. Gasqueta and H. Dumonb

,aLaboratoire de Parasitologie, Hygiène et Zoologie, Faculté de Pharmacie, 27 Bd. Jean Moulin, 13385 Marseille Cedex 05; ,bLaboratoire de Parasitologie, Faculté de Medecine; ,cService de Pédiatrie Pr. Garnier, Hôpital Nord, Marseille, France

Amphotericin B susceptibility was measured by a flow cytometric membrane potential assay in Leishmania infantum promastigotes isolated from 11 immunocompetent children treated with liposomal amphotericin B and 19 HIV-infected young adults treated with intralipid amphotericin B. Susceptibility levels were measured by the 90% inhibitory concentrations (IC 90) representing the concentrations of drug that induced a 90% decrease in membrane potential compared with the control culture. In immunocompetent children, treatment was fully effective whatever the susceptibility of isolates to amphotericin B. In immunocompromised adults, on the contrary, unresponsiveness and relapses could be observed in all cases and IC 90 increased in the course of successive treatments: a decrease of amphotericin B susceptibility in both promastigote and amastigote forms could be observed in a patient who had six relapses. These results suggest that the success of amphotericin B treatment depends greatly on patient immunity status, and indicate that successive relapses could enhance emergence of amphotericin B resistant isolates. The results demonstrate that the flow cytometric membrane potential assay can be used as an easy and reliable tool for studying the evolution of interactions between amphotericin B and the parasite membrane during long-term treatments.

* Corresponding author. Tel: +33-04-91-83-55-44; Fax: +33-04-91-80-26-12.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Clin. Microbiol. Rev.Home page
J. Alvar, P. Aparicio, A. Aseffa, M. Den Boer, C. Canavate, J.-P. Dedet, L. Gradoni, R. Ter Horst, R. Lopez-Velez, and J. Moreno
The Relationship between Leishmaniasis and AIDS: the Second 10 Years
Clin. Microbiol. Rev., April 1, 2008; 21(2): 334 - 359.
[Abstract] [Full Text] [PDF]

Home page
 J Antimicrob ChemotherHome page
J. Collazos
Comment on: Efficacy of liposomal amphotericin B for secondary prophylaxis of visceral leishmaniasis in HIV-infected patients
J. Antimicrob. Chemother., February 1, 2008; 61(2): 466 - 467.
[Full Text] [PDF]

Home page
 Clin. Microbiol. Rev.Home page
S. L. Croft, S. Sundar, and A. H. Fairlamb
Drug Resistance in Leishmaniasis
Clin. Microbiol. Rev., January 1, 2006; 19(1): 111 - 126.
[Abstract] [Full Text] [PDF]

Home page
 Antimicrob. Agents Chemother.Home page
H. I. Al-Mohammed, M. L. Chance, and P. A. Bates
Production and Characterization of Stable Amphotericin-Resistant Amastigotes and Promastigotes of Leishmania mexicana
Antimicrob. Agents Chemother., August 1, 2005; 49(8): 3274 - 3280.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.