Journal of Antimicrobial Chemotherapy (1999) 44, 27-31
© 1999 The British Society for Antimicrobial Chemotherapy
Rhodamine 6G efflux for the detection of CDR1-overexpressing azole-resistant Candida albicans strains
a The Second Department of Internal Medicine, Nagasaki University School of Medicine, 1-7-1 Sakamoto Nagasaki 852, Japan; b Anti-Infectives Research Departments, Janssen Research Foundation, Turnhoutseweg 30, B-2340 Beerse, Belgium; c Institut de Microbiologie, Centre Hospitalier Universitaire Vaudois, 1011 Lausanne, Switzerland
We investigated the drug efflux mechanism in azole-resistant strains of Candida albicans using rhodamine 6G (R6G). No significant differences in R6G uptake were observed between azole-sensitive B2630 (9.02 ± 0.02 nmol/108 cells) and azole-resistant B67081 (8.86 ± 0.03 nmol/108 cells) strains incubated in glucose-free phosphate buffered saline. A significantly higher R6G efflux (2.0 ± 0.21 nmol/108 cells) was noted in the azole-resistant strain (B67081) when glucose was added, compared with that in the sensitive strain B2630 (0.23 ± 0.14 nmol/108 cells). A fluconazole-resistant strain C40 that expressed the benomyl resistance gene (CaMDR) also showed a low R6G efflux (0.16 ± 0.06 nmol/108 cells) as did the sensitive strains. Accumulation of R6G in growing C. albicans cells was inversely correlated with the level of CDR1 mRNA expression. Our data also suggest that measurement of intracellular accumulation of R6G is a useful method for identification of azole-resistant strains due to CDR1-expressed drug efflux pump.
* Corresponding author. Tel: +81-95-849-7276; Fax: +81-95-849-7285.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  S. Tsao, F. Rahkhoodaee, and M. Raymond Relative Contributions of the Candida albicans ABC Transporters Cdr1p and Cdr2p to Clinical Azole Resistance Antimicrob. Agents Chemother., April 1, 2009; 53(4): 1344 - 1352. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. G. Oliver, P. M. Silver, C. Marie, S. J. Hoot, S. E. Leyde, and T. C. White Tetracycline alters drug susceptibility in Candida albicans and other pathogenic fungi Microbiology, March 1, 2008; 154(3): 960 - 970. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H.-F. Tsai, A. A. Krol, K. E. Sarti, and J. E. Bennett Candida glabrata PDR1, a Transcriptional Regulator of a Pleiotropic Drug Resistance Network, Mediates Azole Resistance in Clinical Isolates and Petite Mutants. Antimicrob. Agents Chemother., April 1, 2006; 50(4): 1384 - 1392. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Vandeputte, G. Larcher, T. Berges, G. Renier, D. Chabasse, and J.-P. Bouchara Mechanisms of Azole Resistance in a Clinical Isolate of Candida tropicalis Antimicrob. Agents Chemother., November 1, 2005; 49(11): 4608 - 4615. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H.-F. Tsai, M. Bard, K. Izumikawa, A. A. Krol, A. M. Sturm, N. T. Culbertson, C. A. Pierson, and J. E. Bennett Candida glabrata erg1 Mutant with Increased Sensitivity to Azoles and to Low Oxygen Tension Antimicrob. Agents Chemother., July 1, 2004; 48(7): 2483 - 2489. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Shukla, S. V. Ambudkar, and R. Prasad Substitution of threonine-1351 in the multidrug transporter Cdr1p of Candida albicans results in hypersusceptibility to antifungal agents and threonine-1351 is essential for synergic effects of calcineurin inhibitor FK520 J. Antimicrob. Chemother., July 1, 2004; 54(1): 38 - 45. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Mukhopadhyay, T. Prasad, P. Saini, T. J. Pucadyil, A. Chattopadhyay, and R. Prasad Membrane Sphingolipid-Ergosterol Interactions Are Important Determinants of Multidrug Resistance in Candida albicans Antimicrob. Agents Chemother., May 1, 2004; 48(5): 1778 - 1787. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Brun, T. Berges, P. Poupard, C. Vauzelle-Moreau, G. Renier, D. Chabasse, and J.-P. Bouchara Mechanisms of Azole Resistance in Petite Mutants of Candida glabrata Antimicrob. Agents Chemother., May 1, 2004; 48(5): 1788 - 1796. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Sendid, T. Jouault, R. Coudriau, D. Camus, F. Odds, M. Tabouret, and D. Poulain Increased Sensitivity of Mannanemia Detection Tests by Joint Detection of {alpha}- and {beta}-Linked Oligomannosides during Experimental and Human Systemic Candidiasis J. Clin. Microbiol., January 1, 2004; 42(1): 164 - 171. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Shukla, P. Saini, Smriti, S. Jha, S. V. Ambudkar, and R. Prasad Functional Characterization of Candida albicans ABC Transporter Cdr1p Eukaryot. Cell, December 1, 2003; 2(6): 1361 - 1375. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Pinjon, G. P. Moran, C. J. Jackson, S. L. Kelly, D. Sanglard, D. C. Coleman, and D. J. Sullivan Molecular Mechanisms of Itraconazole Resistance in Candida dubliniensis Antimicrob. Agents Chemother., August 1, 2003; 47(8): 2424 - 2437. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Kohli, N. Smriti, K. Mukhopadhyay, A. Rattan, and R. Prasad In Vitro Low-Level Resistance to Azoles in Candida albicans Is Associated with Changes in Membrane Lipid Fluidity and Asymmetry Antimicrob. Agents Chemother., April 1, 2002; 46(4): 1046 - 1052. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Nakamura, M. Niimi, K. Niimi, A. R. Holmes, J. E. Yates, A. Decottignies, B. C. Monk, A. Goffeau, and R. D. Cannon Functional Expression of Candida albicans Drug Efflux Pump Cdr1p in a Saccharomyces cerevisiae Strain Deficient in Membrane Transporters Antimicrob. Agents Chemother., December 1, 2001; 45(12): 3366 - 3374. [Abstract] [Full Text] [PDF]
|
|