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Journal of Antimicrobial Chemotherapy (1999) 43, 829-832
© 1999 The British Society for Antimicrobial Chemotherapy

Brief report

Use of a clinical Escherichia coli isolate expressing lux genes to study the antimicrobial pharmacodynamics of moxifloxacin

Vyvyan Salisburya,*, Andreas Pfoestla, Herbert Wiesinger-Mayra, Roger Lewisa, Karen E. Bowkerb and Alasdair P. MacGowanb

a Department of Biological and Biomedical Science, University of the West of England, Bristol BS16 1QT; b Bristol Centre for Antimicrobial Research and Evaluation, Department of Medical Microbiology, Southmead Hospital, Bristol BS10 5NB, UK

Escherichia coli isolate 16906 expressing luxgenes was used for real-time monitoring of moxifloxacin effects on bacterial metabolism compared with effects on cell replication. Viable counts showed concentration-dependent killing by moxifloxacin; real-time measurement of bioluminescence on the same cultures showed metabolic activity over 54 h, but with greater inhibition at 1x MIC than with higher MIC multiples. Post-antibiotic effect was longer when determined using bioluminescence than by viable counts. The control-related effective regrowth time was consistent with both methods. Bioluminescent bacteria provide a rapid and sensitive means for measuring antimicrobial effects on bacterial metabolism.

* Corresponding author. Tel: +44-117-9656261; Fax: +44-117-9763871; E-mail: vyv.salisbury{at}uwe.ac.uk


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